Death signaling pathway induced by pyrrolidine dithiocarbamate-Cu2+ complex in the cultured rat cortical astrocytes

Sung Ho Chen, Shing Hwa Liu, Yu Chih Liang, Jen Kun Lin, Shoei Yn Lin-Shiau

研究成果: 雜誌貢獻文章

52 引文 (Scopus)

摘要

The chelating and antioxidant effects of pyrrolidine dithiocarbamate (PDTC) have been investigated extensively for preventing cell death induced by different insults. However, the toxic effects of PDTC have been studied only recently and fewer studies on the toxic effects on astrocytes have been reported. In our study, we demonstrated that both PDTC and Cu2+ alone were rated as only weakly toxic in inducing cell death in cortical astrocytes with IC50 of 300 μM and 180 μM, respectively. However, PDTC and Cu2+ in the complex form markedly potentiated with each other by about 1,000-fold with IC50 of 0.3 μM PDTC plus 10 μM Cu2+. Other metals at concentrations of 3-10 μM (VO45+, Cr6+, Mn2+, Fe2+, Co2, Ni2+, Zn2+, Pb2+, Bi2+, Ba2+, UO2+, Cs+, SeO42-, La3+) had no such potentiating effects on PDTC. Changes in morphology (nuclear condensation), apoptotic body formation, and hypodiploidity of DNA suggested that the PDTC-Cu2+ complex induced cell death through an apoptotic process. Further studies showed that the PDTC-Cu2+ complex decreased mitochondrial membrane potential, increased hydrogen peroxide production, and depleted GSH contents. After the increased oxidative stress, PDTC-Cu2+ complex differentially activated JNKs, ERK, p38 and caspase 3, which caused PARP degradation in a time-dependent manner. All these effects were consistent with the increased cellular Cu contents. The nonpermeable copper-specific chelator bathocuproine disulfonate (BCPS), but not the permeable Cu2+ chelator neocuproine, abolished all the observed effects. Antioxidants (N-acetylcysteine [NAC], vitamin C), catalase, and Cu2+-binding proteins (albumin, hemoglobin, and higher serum) reduced the cytotoxic effects of PDTC-Cu2+ complex. We concluded that the death signaling pathway of PDTC-Cu2+ complex was mediated by oxidative stress and subsequent JNK activation. These findings imply that PDTC, a widely used pesticide and medicine that is capable of penetrating the blood-brain barrier, may cause neurotoxicity through astrocyte dysfunction. (C) 2000 Wiley-Liss, Inc.
原文英語
頁(從 - 到)249-261
頁數13
期刊GLIA
31
發行號3
DOIs
出版狀態已發佈 - 2000
對外發佈Yes

指紋

Astrocytes
Poisons
Cell Death
Chelating Agents
Inhibitory Concentration 50
pyrrolidine dithiocarbamic acid
Oxidative Stress
Antioxidants
Mitochondrial Membrane Potential
Acetylcysteine
Blood-Brain Barrier
Pesticides
Caspase 3
Catalase
Hydrogen Peroxide
Ascorbic Acid
Copper
Albumins
Carrier Proteins
Hemoglobins

ASJC Scopus subject areas

  • Immunology

引用此文

Death signaling pathway induced by pyrrolidine dithiocarbamate-Cu2+ complex in the cultured rat cortical astrocytes. / Chen, Sung Ho; Liu, Shing Hwa; Liang, Yu Chih; Lin, Jen Kun; Lin-Shiau, Shoei Yn.

於: GLIA, 卷 31, 編號 3, 2000, p. 249-261.

研究成果: 雜誌貢獻文章

Chen, Sung Ho ; Liu, Shing Hwa ; Liang, Yu Chih ; Lin, Jen Kun ; Lin-Shiau, Shoei Yn. / Death signaling pathway induced by pyrrolidine dithiocarbamate-Cu2+ complex in the cultured rat cortical astrocytes. 於: GLIA. 2000 ; 卷 31, 編號 3. 頁 249-261.
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abstract = "The chelating and antioxidant effects of pyrrolidine dithiocarbamate (PDTC) have been investigated extensively for preventing cell death induced by different insults. However, the toxic effects of PDTC have been studied only recently and fewer studies on the toxic effects on astrocytes have been reported. In our study, we demonstrated that both PDTC and Cu2+ alone were rated as only weakly toxic in inducing cell death in cortical astrocytes with IC50 of 300 μM and 180 μM, respectively. However, PDTC and Cu2+ in the complex form markedly potentiated with each other by about 1,000-fold with IC50 of 0.3 μM PDTC plus 10 μM Cu2+. Other metals at concentrations of 3-10 μM (VO45+, Cr6+, Mn2+, Fe2+, Co2, Ni2+, Zn2+, Pb2+, Bi2+, Ba2+, UO2+, Cs+, SeO42-, La3+) had no such potentiating effects on PDTC. Changes in morphology (nuclear condensation), apoptotic body formation, and hypodiploidity of DNA suggested that the PDTC-Cu2+ complex induced cell death through an apoptotic process. Further studies showed that the PDTC-Cu2+ complex decreased mitochondrial membrane potential, increased hydrogen peroxide production, and depleted GSH contents. After the increased oxidative stress, PDTC-Cu2+ complex differentially activated JNKs, ERK, p38 and caspase 3, which caused PARP degradation in a time-dependent manner. All these effects were consistent with the increased cellular Cu contents. The nonpermeable copper-specific chelator bathocuproine disulfonate (BCPS), but not the permeable Cu2+ chelator neocuproine, abolished all the observed effects. Antioxidants (N-acetylcysteine [NAC], vitamin C), catalase, and Cu2+-binding proteins (albumin, hemoglobin, and higher serum) reduced the cytotoxic effects of PDTC-Cu2+ complex. We concluded that the death signaling pathway of PDTC-Cu2+ complex was mediated by oxidative stress and subsequent JNK activation. These findings imply that PDTC, a widely used pesticide and medicine that is capable of penetrating the blood-brain barrier, may cause neurotoxicity through astrocyte dysfunction. (C) 2000 Wiley-Liss, Inc.",
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T1 - Death signaling pathway induced by pyrrolidine dithiocarbamate-Cu2+ complex in the cultured rat cortical astrocytes

AU - Chen, Sung Ho

AU - Liu, Shing Hwa

AU - Liang, Yu Chih

AU - Lin, Jen Kun

AU - Lin-Shiau, Shoei Yn

PY - 2000

Y1 - 2000

N2 - The chelating and antioxidant effects of pyrrolidine dithiocarbamate (PDTC) have been investigated extensively for preventing cell death induced by different insults. However, the toxic effects of PDTC have been studied only recently and fewer studies on the toxic effects on astrocytes have been reported. In our study, we demonstrated that both PDTC and Cu2+ alone were rated as only weakly toxic in inducing cell death in cortical astrocytes with IC50 of 300 μM and 180 μM, respectively. However, PDTC and Cu2+ in the complex form markedly potentiated with each other by about 1,000-fold with IC50 of 0.3 μM PDTC plus 10 μM Cu2+. Other metals at concentrations of 3-10 μM (VO45+, Cr6+, Mn2+, Fe2+, Co2, Ni2+, Zn2+, Pb2+, Bi2+, Ba2+, UO2+, Cs+, SeO42-, La3+) had no such potentiating effects on PDTC. Changes in morphology (nuclear condensation), apoptotic body formation, and hypodiploidity of DNA suggested that the PDTC-Cu2+ complex induced cell death through an apoptotic process. Further studies showed that the PDTC-Cu2+ complex decreased mitochondrial membrane potential, increased hydrogen peroxide production, and depleted GSH contents. After the increased oxidative stress, PDTC-Cu2+ complex differentially activated JNKs, ERK, p38 and caspase 3, which caused PARP degradation in a time-dependent manner. All these effects were consistent with the increased cellular Cu contents. The nonpermeable copper-specific chelator bathocuproine disulfonate (BCPS), but not the permeable Cu2+ chelator neocuproine, abolished all the observed effects. Antioxidants (N-acetylcysteine [NAC], vitamin C), catalase, and Cu2+-binding proteins (albumin, hemoglobin, and higher serum) reduced the cytotoxic effects of PDTC-Cu2+ complex. We concluded that the death signaling pathway of PDTC-Cu2+ complex was mediated by oxidative stress and subsequent JNK activation. These findings imply that PDTC, a widely used pesticide and medicine that is capable of penetrating the blood-brain barrier, may cause neurotoxicity through astrocyte dysfunction. (C) 2000 Wiley-Liss, Inc.

AB - The chelating and antioxidant effects of pyrrolidine dithiocarbamate (PDTC) have been investigated extensively for preventing cell death induced by different insults. However, the toxic effects of PDTC have been studied only recently and fewer studies on the toxic effects on astrocytes have been reported. In our study, we demonstrated that both PDTC and Cu2+ alone were rated as only weakly toxic in inducing cell death in cortical astrocytes with IC50 of 300 μM and 180 μM, respectively. However, PDTC and Cu2+ in the complex form markedly potentiated with each other by about 1,000-fold with IC50 of 0.3 μM PDTC plus 10 μM Cu2+. Other metals at concentrations of 3-10 μM (VO45+, Cr6+, Mn2+, Fe2+, Co2, Ni2+, Zn2+, Pb2+, Bi2+, Ba2+, UO2+, Cs+, SeO42-, La3+) had no such potentiating effects on PDTC. Changes in morphology (nuclear condensation), apoptotic body formation, and hypodiploidity of DNA suggested that the PDTC-Cu2+ complex induced cell death through an apoptotic process. Further studies showed that the PDTC-Cu2+ complex decreased mitochondrial membrane potential, increased hydrogen peroxide production, and depleted GSH contents. After the increased oxidative stress, PDTC-Cu2+ complex differentially activated JNKs, ERK, p38 and caspase 3, which caused PARP degradation in a time-dependent manner. All these effects were consistent with the increased cellular Cu contents. The nonpermeable copper-specific chelator bathocuproine disulfonate (BCPS), but not the permeable Cu2+ chelator neocuproine, abolished all the observed effects. Antioxidants (N-acetylcysteine [NAC], vitamin C), catalase, and Cu2+-binding proteins (albumin, hemoglobin, and higher serum) reduced the cytotoxic effects of PDTC-Cu2+ complex. We concluded that the death signaling pathway of PDTC-Cu2+ complex was mediated by oxidative stress and subsequent JNK activation. These findings imply that PDTC, a widely used pesticide and medicine that is capable of penetrating the blood-brain barrier, may cause neurotoxicity through astrocyte dysfunction. (C) 2000 Wiley-Liss, Inc.

KW - Antioxidant

KW - Apoptosis

KW - BCPS

KW - Caspase

KW - Cu

KW - MAP kinase

KW - Oxidative stress

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