DC-SIGN mediates avian H5N1 influenza virus infection in cis and in trans

Sheng Fan Wang, Jason C. Huang, Yuan Ming Lee, Shih Jen Liu, Yu Jiun Chan, Yat Pang Chau, Pele Chong, Yi Ming Arthur Chen

研究成果: 雜誌貢獻文章同行評審

65 引文 斯高帕斯(Scopus)

摘要

DC-SIGN, a C-type lectin receptor expressed in dendritic cells (DCs), has been identified as a receptor for human immunodeficiency virus type 1, hepatitis C virus, Ebola virus, cytomegalovirus, dengue virus, and the SARS coronavirus. We used H5N1 pseudotyped and reverse-genetics (RG) virus particles to study their ability to bind with DC-SIGN. Electronic microscopy and functional assay results indicate that pseudotyped viruses containing both HA and NA proteins express hemagglutination and are capable of infecting cells expressing α-2,3-linked sialic acid receptors. Results from a capture assay show that DC-SIGN-expressing cells (including B-THP-1/DC-SIGN and T-THP-1/DC-SIGN) and peripheral blood dendritic cells are capable of transferring H5N1 pseudotyped and RG virus particles to target cells; this action can be blocked by anti-DC-SIGN monoclonal antibodies. In summary, (a) DC-SIGN acts as a capture or attachment molecule for avian H5N1 virus, and (b) DC-SIGN mediates infections in cis and in trans.
原文英語
頁(從 - 到)561-566
頁數6
期刊Biochemical and Biophysical Research Communications
373
發行號4
DOIs
出版狀態已發佈 - 九月 5 2008
對外發佈

ASJC Scopus subject areas

  • 生物物理學
  • 生物化學
  • 分子生物學
  • 細胞生物學

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