D-Aspartate (D-Asp) is found in prolactin (PRL)-containing cells of the rat anterior pituitary gland [Lee et al., Brain Res. 838, 193-199, 1999]. In order to determine whether D-Asp is actually produced by the anterior pituitary gland and whether it plays a physiological role in PRL function, a PRL-secreting clonal strain of rat pituitary tumor cells (GH3) was employed in this study. HPLC analysis and immunocytochemical staining detected the presence and synthesis of D-Asp in the cytoplasm of these cells. In addition, thyrotropin-releasing hormone-stimulated PRL secretion was increased in a dose-dependent fashion by D-Asp from these cells. These results suggest that the anterior pituitary gland synthesizes D-Asp and that D-Asp acts as a messenger in this gland. (C) 2000 Academic Press.
|頁（從 - 到）||1143-1147|
|期刊||Biochemical and Biophysical Research Communications|
|出版狀態||已發佈 - 十月 5 2000|
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