摘要
D-Aspartate (D-Asp) is found in prolactin (PRL)-containing cells of the rat anterior pituitary gland [Lee et al., Brain Res. 838, 193-199, 1999]. In order to determine whether D-Asp is actually produced by the anterior pituitary gland and whether it plays a physiological role in PRL function, a PRL-secreting clonal strain of rat pituitary tumor cells (GH3) was employed in this study. HPLC analysis and immunocytochemical staining detected the presence and synthesis of D-Asp in the cytoplasm of these cells. In addition, thyrotropin-releasing hormone-stimulated PRL secretion was increased in a dose-dependent fashion by D-Asp from these cells. These results suggest that the anterior pituitary gland synthesizes D-Asp and that D-Asp acts as a messenger in this gland. (C) 2000 Academic Press.
原文 | 英語 |
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頁(從 - 到) | 1143-1147 |
頁數 | 5 |
期刊 | Biochemical and Biophysical Research Communications |
卷 | 276 |
發行號 | 3 |
DOIs | |
出版狀態 | 已發佈 - 10月 5 2000 |
ASJC Scopus subject areas
- 生物化學
- 生物物理學
- 分子生物學