Cytotoxicity of silicone oil on cultivated human corneal endothelium

C. S. Yang, K. H. Chen, Wen-Ming Hsu, Y. S. Li

研究成果: 雜誌貢獻文章同行評審

19 引文 斯高帕斯(Scopus)

摘要

Background and Objective: To investigate the cytotoxic effects of silicone oil on the cultivated human corneal endothelial cells (CEs). Methods: We cultured human CE and passed them in insert wells that allowed the apical side of CE monolayer in contact with the silicone oil. The tested silicone oils were of two different viscosities, 1000 and 5000 centistoke (CS). MTS proliferation bioassay and calcein-acetoxymethyl ester (CAM)-ethidium homodimer staining were performed to evaluate cell viability after CEs were co-cultured with silicone oils for 48h. Apoptosis of CEs was evaluated by TdT-mediated dUTP nick-end labelling (TUNEL) stain. Results: The MTS bioassay showed that contact of silicone oil inhibited CE proliferation. The higher viscosity (5000CS) silicone oil suppressed cell cycling significantly more than the lower viscosity (1000CS) silicone oil. CAM-ethidium homodimer staining revealed CE death, 9.1±0.1% (1000 CS silicone oil) and 41.6±0.4% (5000 CS), but apoptosis played only minor role in silicone oil toxicity, 1.7±0.1% (1000 CS silicone oil) and 9.4±0.1% (5000 CS). Conclusions: Silicone oil is cytotoxic to cultivated human CEs. Avoiding the forward migration of silicone oil to the anterior chamber and corneal CE contact is critical in preventing silicone oil-associated keratopathy. Silicone oil should be removed as early as possible once the goal of tamponade has been achieved.

原文英語
頁(從 - 到)282-288
頁數7
期刊Eye
22
發行號2
DOIs
出版狀態已發佈 - 一月 1 2008
對外發佈Yes

ASJC Scopus subject areas

  • Ophthalmology
  • Arts and Humanities(all)

指紋 深入研究「Cytotoxicity of silicone oil on cultivated human corneal endothelium」主題。共同形成了獨特的指紋。

引用此