Cytotoxicity assessment of nanoparticles and polycyclic aromatic hydrocarbons exhausted from motorcycle

S. M. Chien, Yuh Jeen Huang, C. Y. Chuang, I. L. Hsiao

研究成果: 書貢獻/報告類型會議貢獻

摘要

The cell cytotoxicity of nanoparticles (NPs) and particle-bound polycyclic aromatic hydrocarbons (p-PAHs) in emission of a four-stroke motorcycle (4-st/mc) was studied. Cell cytotoxicity was evaluated by detecting the cell viability rate (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide, MTT assay) of carcinomic human airway alveolar epithelial cells (A549) and the content of cytokine (IL-8) in the mucus of A549 after exposing to NPs and p-PAHs. In experiment, as the speed accelerated (15 km/h and 30 km/h), the concentration of high molecular weight PAHs (HMW-PAHs) increased. According to the result of MTT assay, the cell viability rate of A549 exposed to the NPs exhausted from idle condition was lower than that from 15 km/h and 30 km/h speed. However, the cell viability rate of A549 stimulated by p-PAHs exhausted from the 30 km/h condition was 15.9%, which was lower than 31.4% (idle) and 20.2% (15 km/h). It means the p-PAHs might be the main factor to induce the death of cell in the high speed condition. Moreover, p-PAHs exhausted from the idle condition could cause more serious inflammation in the A549 cells than that from other speeds.
原文英語
主出版物標題Nanotechnology 2010
主出版物子標題Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
頁面587-590
頁數4
3
出版狀態已發佈 - 2010
對外發佈Yes
事件Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 - Anaheim, CA, 美国
持續時間: 六月 21 2010六月 24 2010

會議

會議Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
國家美国
城市Anaheim, CA
期間6/21/106/24/10

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering
  • Electrical and Electronic Engineering

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