1 引文 斯高帕斯(Scopus)

摘要

Hydrangea angustipetala Hayata (Saxifragaceae) is an anti-malaria folk medicine and grows in mountains of northern Taiwan. The leaves of H. angustipetala were extracted with 70% acetone and column chromatography was combined with cytotoxic bioassay-guided fractionation to isolate the cytotoxic compounds. The Diaion HP-20 column 40% MeOH eluted fraction (D40M) of H. angustipetala inhibited the growth of P-388D1 cells in vitro and prolonged the survival days of P-388D1-bearing CDF1 mice. Furthermore, (+)-febrifugine (1) and trans-3-p-coumaroylquinic acid (2) were isolated from the D40M, and both compounds induced apoptosis in AGS and SNU-1 cells. However, the cytotoxicity of 1 was stronger than 2 and IC50 values of 1 were 0.17 and 0.05 μg/ml in AGS and SNU-1 cells after treatment for 48 h, respectively. The two compounds both induced decreases in PARP and pro-caspase 3 of AGS and SNU-1 cells, and pre-treatment of cells with a caspase-3-specific inhibitor reduced cytotoxicity by 1 and 2 in AGS and SNU-1 cells. We suggested that the activation of caspase-3 may provide a mechanistic explanation for their (1 and 2) cytotoxic effects in AGS and SNU-1 cells. (+)-Febrifugine was a good lead compound as development an anticancer drug.

原文英語
頁(從 - 到)45-51
頁數7
期刊Botanical Studies
51
發行號1
出版狀態已發佈 - 一月 2010

ASJC Scopus subject areas

  • 植物科學

指紋

深入研究「Cytotoxic constituents of Hydrangea angustipetala on human gastric carcinoma cells」主題。共同形成了獨特的指紋。

引用此