Cytotoxic activity of sesquiterpenoids from Atractylodes ovata on leukemia cell lines

Ching Chiung Wang, Lih Geeng Chen, Ling-Ling Yang

研究成果: 雜誌貢獻文章同行評審

62 引文 斯高帕斯(Scopus)

摘要

The rhizome of Atractylodes ovata (Bai Zhu in Chinese) is a widely used traditional Chinese herb in Taiwan as a tonic agent. In this paper, four sesquiterpenoids, namely atractylon, and atractylenolides I, II, and III, were isolated from the n-hexane extract of A. ovata and were evaluated for cytotoxic effects in vitro. Atractylon significantly inhibited the growth of human leukemia cell line HL-60 and mouse leukemia cell line P-388, and showed low cytotoxicity against primary cultures of normal human peripheral blood mononuclear cells at 15 μg/ml for 12 h. Atractylon had a dose-dependent antiproliferative effect on the two tumor cell lines. In accordance with DNA fragment increases and PARP protein decreases, atractylon at 15 μg/ml for 6 h induced apoptosis in HL-60 cells. Moreover, atractylon inhibited the viability of P-388 cells and induced apoptosis after 15 μg/ml treatment for 12 h in an in vitro assay. However, atractylenolide 1 at 30 μg/ml for 12 h also induced apoptosis in HL-60 and P-388 cells, but atractylenolides II and III showed no significant inhibition effects on tumor cell growth. As the above results suggested, atractylon and atractylenolide I were the major cytotoxic principle constituents of A. ovata on leukemia cell lines.

原文英語
頁(從 - 到)204-208
頁數5
期刊Planta Medica
68
發行號3
DOIs
出版狀態已發佈 - 2002

ASJC Scopus subject areas

  • 植物科學
  • 藥物發現
  • 有機化學
  • 藥理

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