Cytolethal Distending Toxin Enhances Radiosensitivity in Prostate Cancer Cells by Regulating Autophagy

Hwai Jeng Lin, Hsin Ho Liu, Chia Der Lin, Min Chuan Kao, Yu An Chen, Chuan Chiang-Ni, Zhi Pei Jiang, Mei Zi Huang, Chun Jung Lin, U. Ging Lo, Li Chiung Lin, Cheng Kuo Lai, Ho Lin, Jer Tsong Hsieh, Cheng Hsun Chiu, Chih Ho Lai

研究成果: 雜誌貢獻文章同行評審

12 引文 斯高帕斯(Scopus)

摘要

Cytolethal distending toxin (CDT) produced by Campylobacter jejuni contains three subunits: CdtA, CdtB, and CdtC. Among these three toxin subunits, CdtB is the toxic moiety of CDT with DNase I activity, resulting in DNA double-strand breaks (DSB) and, consequently, cell cycle arrest at the G2/M stage and apoptosis. Radiation therapy is an effective modality for the treatment of localized prostate cancer (PCa). However, patients often develop radioresistance. Owing to its particular biochemical properties, we previously employed CdtB as a therapeutic agent for sensitizing radioresistant PCa cells to ionizing radiation (IR). In this study, we further demonstrated that CDT suppresses the IR-induced autophagy pathway in PCa cells by attenuating c-Myc expression and therefore sensitizes PCa cells to radiation. We further showed that CDT prevents the formation of autophagosomes via decreased high-mobility group box 1 (HMGB1) expression and the inhibition of acidic vesicular organelle (AVO) formation, which are associated with enhanced radiosensitivity in PCa cells. The results of this study reveal the detailed mechanism of CDT for the treatment of radioresistant PCa.

原文英語
文章編號223
期刊Frontiers in cellular and infection microbiology
7
發行號JUN
DOIs
出版狀態已發佈 - 六月 8 2017

ASJC Scopus subject areas

  • 微生物學
  • 免疫學
  • 微生物學(醫學)
  • 傳染性疾病

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