Cytokine gene-modulated dendritic cells protect against allergic airway inflammation by inducing IL-10+ IFN-γ CD4+ T cells

C. Y. Hsu, S. J. Leu, B. L. Chiang, H. E. Liu, H. C. Su, Y. L. Lee

研究成果: 雜誌貢獻文章

13 引文 (Scopus)

摘要

Asthma is characterized by allergen-induced airway inflammation orchestrated by Th2 cells. Dendritic cells (DCs) were found to efficiently prime naive T-helper cells. Thus, modification of DC function may be used as an ideal tool to treat allergic asthma by changing CD4+ T-cell differentiation or suppressing Th2 development. In this study, we examined whether a DC-based vaccine can be applied to DCs modified with interleukin (IL)-10-and IL-12-expressing adenoviruses to prevent ovalbumin (OVA)-induced asthma in mice. Herein, we show that these modified DCs efficiently moderated the characteristics of asthma, including expressions of OVA-specific antibodies, airway hyperresponsiveness, eosinophilic airway inflammation, and Th2 cytokines production. Additionally, IL-10 and IL-12 gene-modified DCs enhanced the development of both T-helper type 1 (Th1) and IL-10 IFN-γ (interferon-γ) double-positive T cells in vivo. In vitro-generated OVA-specific IL-10 IFN-γ CD4+ T cells inhibited the proliferation of naive CD4+ T cells, and this suppressive effect was a cell contact-dependent mechanism. Furthermore, we showed that combined cytokine-modulated DCs could alleviate established allergic airway inflammation. Taken together, these results suggest that IL-10 and IL-12 gene-modulated DCs are effective in suppressing asthmatic airway inflammation through both immune deviation and immune suppression and are a potential therapeutic approach for asthma.
原文英語
頁(從 - 到)1011-1021
頁數11
期刊Gene Therapy
17
發行號8
DOIs
出版狀態已發佈 - 八月 2010

指紋

Interleukin-10
Dendritic Cells
Interferons
Cytokines
Inflammation
T-Lymphocytes
Asthma
Genes
Ovalbumin
Interleukin-12
Th2 Cells
Helper-Inducer T-Lymphocytes
Adenoviridae
Allergens
Cell Differentiation
Vaccines
Cell Proliferation
Antibodies

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Genetics

引用此文

Cytokine gene-modulated dendritic cells protect against allergic airway inflammation by inducing IL-10+ IFN-γ CD4+ T cells. / Hsu, C. Y.; Leu, S. J.; Chiang, B. L.; Liu, H. E.; Su, H. C.; Lee, Y. L.

於: Gene Therapy, 卷 17, 編號 8, 08.2010, p. 1011-1021.

研究成果: 雜誌貢獻文章

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