Current status of MALDI-TOF mass spectrometry in clinical microbiology

Tsung Yun Hou, Chuan Chiang-Ni, Shih Hua Teng

研究成果: 雜誌貢獻回顧型文獻同行評審

40 引文 斯高帕斯(Scopus)

摘要

Mass spectrometry (MS) is a type of analysis used to determine what molecules make up a sample, based on the mass spectrum that are created by the ions. Mass spectrometers are able to perform traditional target analyte identification and quantitation; however, they may also be used within a clinical setting for the rapid identification of bacteria. The causative agent in sepsis is changed over time, and clinical decisions affecting the management of infections are often based on the outcomes of bacterial identification. Therefore, it is essential that such identifications are performed quickly and interpreted correctly. Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometer is one of the most popular MS instruments used in biology, due to its rapid and precise identification of genus and species of an extensive range of Gram-negative and -positive bacteria. Microorganism identification by Mass spectrometry is based on identifying a characteristic spectrum of each species and then matched with a large database within the instrument. The present review gives a contemporary perspective on the challenges and opportunities for bacterial identification as well as a written report of how technological innovation has advanced MS. Future clinical applications will also be addressed, particularly the use of MALDI-TOF MS in the field of microbiology for the identification and the analysis of antibiotic resistance.

原文英語
頁(從 - 到)404-414
頁數11
期刊Journal of Food and Drug Analysis
27
發行號2
DOIs
出版狀態已發佈 - 四月 1 2019

ASJC Scopus subject areas

  • Food Science
  • Pharmacology

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