摘要

Background: Traumatic spinal cord injury (SCI) is a major cause of long-term disability. However, therapeutic agents targeting SCI are sorely lacking. The aim of this study was to investigate whether curcumin has neuroprotective effects after SCI in rats. Materials and methods: Studies were performed in 39 male Sprague-Dawley rats after spinal cord hemisection. The animals were randomly divided into three groups: sham, vehicle, and curcumin. The Basso, Beattie, and Bresnahan (BBB) scale was used to evaluate functional outcome. Specimens were tested for histologic, terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL), and immunohistochemical staining. Primary cultured astrocytes were used to test the inhibitory effect of curcumin on glial reactivation. Results: The BBB scores for the affected hindlimb after hemisection were significantly improved in the curcumin-treated group compared with the vehicle group (on d 3 and 7; P <0.001). Immunohistochemistry of NeuN revealed remarkable neuronal loss in the vehicle group after hemisection. In comparison, curcumin significantly protected neurons after SCI (curcumin compared with vehicle; P <0.001). Furthermore, curcumin significantly attenuated apoptosis after SCI (curcumin compared with vehicle; P <0.001). RT-PCR demonstrated that the expression of glial fibrillary acidic protein (GFAP) was significantly inhibited by curcumin. Conclusions: Curcumin inhibited apoptosis and neuron loss, quenched astrocyte activation, and significantly improved neurologic deficit 7 d after spinal cord hemisection. By down-regulating GFAP expression, curcumin seems to attenuate astrocyte reactivation, which may be beneficial for neuronal survival. This is the first report demonstrating the successful treatment of SCI by curcumin.
原文英語
頁(從 - 到)280-289
頁數10
期刊Journal of Surgical Research
166
發行號2
DOIs
出版狀態已發佈 - 四月 2011

指紋

Curcumin
Spinal Cord Injuries
Astrocytes
Glial Fibrillary Acidic Protein
Neuroprotection
Spinal Cord
Apoptosis
Neurons
DNA Nucleotidylexotransferase
Neuroprotective Agents
Hindlimb
Neurologic Manifestations
Neuroglia
Sprague Dawley Rats
Immunohistochemistry

ASJC Scopus subject areas

  • Surgery

引用此文

Curcumin provides neuroprotection after spinal cord injury. / Lin, Muh Shi; Lee, Yi Hsuan; Chiu, Wen Ta; Hung, Kuo Sheng.

於: Journal of Surgical Research, 卷 166, 編號 2, 04.2011, p. 280-289.

研究成果: 雜誌貢獻文章

@article{6ccf1103a22b48d781f18d1976df850a,
title = "Curcumin provides neuroprotection after spinal cord injury",
abstract = "Background: Traumatic spinal cord injury (SCI) is a major cause of long-term disability. However, therapeutic agents targeting SCI are sorely lacking. The aim of this study was to investigate whether curcumin has neuroprotective effects after SCI in rats. Materials and methods: Studies were performed in 39 male Sprague-Dawley rats after spinal cord hemisection. The animals were randomly divided into three groups: sham, vehicle, and curcumin. The Basso, Beattie, and Bresnahan (BBB) scale was used to evaluate functional outcome. Specimens were tested for histologic, terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL), and immunohistochemical staining. Primary cultured astrocytes were used to test the inhibitory effect of curcumin on glial reactivation. Results: The BBB scores for the affected hindlimb after hemisection were significantly improved in the curcumin-treated group compared with the vehicle group (on d 3 and 7; P <0.001). Immunohistochemistry of NeuN revealed remarkable neuronal loss in the vehicle group after hemisection. In comparison, curcumin significantly protected neurons after SCI (curcumin compared with vehicle; P <0.001). Furthermore, curcumin significantly attenuated apoptosis after SCI (curcumin compared with vehicle; P <0.001). RT-PCR demonstrated that the expression of glial fibrillary acidic protein (GFAP) was significantly inhibited by curcumin. Conclusions: Curcumin inhibited apoptosis and neuron loss, quenched astrocyte activation, and significantly improved neurologic deficit 7 d after spinal cord hemisection. By down-regulating GFAP expression, curcumin seems to attenuate astrocyte reactivation, which may be beneficial for neuronal survival. This is the first report demonstrating the successful treatment of SCI by curcumin.",
keywords = "apoptosis, astrocyte primary culture, astrocyte reactivation, BBB scale, curcumin, functional outcome, GFAP, hemisection, neuroprotection, spinal cord injury",
author = "Lin, {Muh Shi} and Lee, {Yi Hsuan} and Chiu, {Wen Ta} and Hung, {Kuo Sheng}",
year = "2011",
month = "4",
doi = "10.1016/j.jss.2009.07.001",
language = "English",
volume = "166",
pages = "280--289",
journal = "Journal of Surgical Research",
issn = "0022-4804",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Curcumin provides neuroprotection after spinal cord injury

AU - Lin, Muh Shi

AU - Lee, Yi Hsuan

AU - Chiu, Wen Ta

AU - Hung, Kuo Sheng

PY - 2011/4

Y1 - 2011/4

N2 - Background: Traumatic spinal cord injury (SCI) is a major cause of long-term disability. However, therapeutic agents targeting SCI are sorely lacking. The aim of this study was to investigate whether curcumin has neuroprotective effects after SCI in rats. Materials and methods: Studies were performed in 39 male Sprague-Dawley rats after spinal cord hemisection. The animals were randomly divided into three groups: sham, vehicle, and curcumin. The Basso, Beattie, and Bresnahan (BBB) scale was used to evaluate functional outcome. Specimens were tested for histologic, terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL), and immunohistochemical staining. Primary cultured astrocytes were used to test the inhibitory effect of curcumin on glial reactivation. Results: The BBB scores for the affected hindlimb after hemisection were significantly improved in the curcumin-treated group compared with the vehicle group (on d 3 and 7; P <0.001). Immunohistochemistry of NeuN revealed remarkable neuronal loss in the vehicle group after hemisection. In comparison, curcumin significantly protected neurons after SCI (curcumin compared with vehicle; P <0.001). Furthermore, curcumin significantly attenuated apoptosis after SCI (curcumin compared with vehicle; P <0.001). RT-PCR demonstrated that the expression of glial fibrillary acidic protein (GFAP) was significantly inhibited by curcumin. Conclusions: Curcumin inhibited apoptosis and neuron loss, quenched astrocyte activation, and significantly improved neurologic deficit 7 d after spinal cord hemisection. By down-regulating GFAP expression, curcumin seems to attenuate astrocyte reactivation, which may be beneficial for neuronal survival. This is the first report demonstrating the successful treatment of SCI by curcumin.

AB - Background: Traumatic spinal cord injury (SCI) is a major cause of long-term disability. However, therapeutic agents targeting SCI are sorely lacking. The aim of this study was to investigate whether curcumin has neuroprotective effects after SCI in rats. Materials and methods: Studies were performed in 39 male Sprague-Dawley rats after spinal cord hemisection. The animals were randomly divided into three groups: sham, vehicle, and curcumin. The Basso, Beattie, and Bresnahan (BBB) scale was used to evaluate functional outcome. Specimens were tested for histologic, terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL), and immunohistochemical staining. Primary cultured astrocytes were used to test the inhibitory effect of curcumin on glial reactivation. Results: The BBB scores for the affected hindlimb after hemisection were significantly improved in the curcumin-treated group compared with the vehicle group (on d 3 and 7; P <0.001). Immunohistochemistry of NeuN revealed remarkable neuronal loss in the vehicle group after hemisection. In comparison, curcumin significantly protected neurons after SCI (curcumin compared with vehicle; P <0.001). Furthermore, curcumin significantly attenuated apoptosis after SCI (curcumin compared with vehicle; P <0.001). RT-PCR demonstrated that the expression of glial fibrillary acidic protein (GFAP) was significantly inhibited by curcumin. Conclusions: Curcumin inhibited apoptosis and neuron loss, quenched astrocyte activation, and significantly improved neurologic deficit 7 d after spinal cord hemisection. By down-regulating GFAP expression, curcumin seems to attenuate astrocyte reactivation, which may be beneficial for neuronal survival. This is the first report demonstrating the successful treatment of SCI by curcumin.

KW - apoptosis

KW - astrocyte primary culture

KW - astrocyte reactivation

KW - BBB scale

KW - curcumin

KW - functional outcome

KW - GFAP

KW - hemisection

KW - neuroprotection

KW - spinal cord injury

UR - http://www.scopus.com/inward/record.url?scp=79952485690&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952485690&partnerID=8YFLogxK

U2 - 10.1016/j.jss.2009.07.001

DO - 10.1016/j.jss.2009.07.001

M3 - Article

C2 - 20018302

AN - SCOPUS:79952485690

VL - 166

SP - 280

EP - 289

JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

IS - 2

ER -