Curcumin-induced heme oxygenase-1 expression plays a negative role for its anti-cancer effect in bladder cancers

Szu Yuan Wu, Ying Ray Lee, Chin Chin Huang, Yi Zhen Li, Yi Sheng Chang, Chu Yi Yang, Jiann Der Wu, Yi Wen Liu

研究成果: 雜誌貢獻文章

9 引文 (Scopus)

摘要

Some phytochemicals with the characteristics of cytotoxicity and anti-metastasis has generated intense interest among the invasive cancer study. Curcumin, one of these anti-cancer phytochemicals, has been reported to induce the cytoprotective enzyme heme oxygenase-1 expression. Since heme oxygenase-1 has been suggested to enhance cancer cell invasion, we investigated the anti-invasive effect of curcumin when heme oxygenase-1 was knocked down in vitro, and the heme oxygenase-1 expression after curcumin treatment in vivo. Curcumin inhibited cell viability and the MMP-2/9 activities of human bladder cancer cells. At 10μM, curcumin inhibited cell viability and cell invasive activity by 15% and 40%, respectively. Ten micrometer curcumin increased the intracellular reactive oxygen species concentration and heme oxygenase-1 protein and mRNA expression in bladder cancer cells. The anti-invasive activity of curcumin was elevated when heme oxygenase-1 was knocked down by siRNA or inhibited by pharmacological inhibitor. In vivo, curcumin induced heme oxygenase-1 protein expression in the lung tissue of murine lung metastasis tumor model and in the bladder tissue of murine orthotopic bladder tumor model. Taken together, our data suggest that curcumin-induced heme oxygenase-1 attenuates the anti-invasive effect of curcumin in cancer therapy, and co-treatment by heme oxygenase-1 inhibitor enhances the anti-invasive activity of curcumin.

原文英語
頁(從 - 到)3530-3536
頁數7
期刊Food and Chemical Toxicology
50
發行號10
DOIs
出版狀態已發佈 - 十月 2012

指紋

heme oxygenase (biliverdin-producing)
Heme Oxygenase-1
Curcumin
curcumin
Urinary Bladder Neoplasms
Neoplasms
Cells
neoplasms
Phytochemicals
metastasis
phytopharmaceuticals
cell viability
Tumors
Cell Survival
urinary bladder neoplasms
antineoplastic activity
lungs
Tissue
Neoplasm Metastasis
Lung

ASJC Scopus subject areas

  • Food Science
  • Toxicology

引用此文

Curcumin-induced heme oxygenase-1 expression plays a negative role for its anti-cancer effect in bladder cancers. / Wu, Szu Yuan; Lee, Ying Ray; Huang, Chin Chin; Li, Yi Zhen; Chang, Yi Sheng; Yang, Chu Yi; Wu, Jiann Der; Liu, Yi Wen.

於: Food and Chemical Toxicology, 卷 50, 編號 10, 10.2012, p. 3530-3536.

研究成果: 雜誌貢獻文章

Wu, Szu Yuan ; Lee, Ying Ray ; Huang, Chin Chin ; Li, Yi Zhen ; Chang, Yi Sheng ; Yang, Chu Yi ; Wu, Jiann Der ; Liu, Yi Wen. / Curcumin-induced heme oxygenase-1 expression plays a negative role for its anti-cancer effect in bladder cancers. 於: Food and Chemical Toxicology. 2012 ; 卷 50, 編號 10. 頁 3530-3536.
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abstract = "Some phytochemicals with the characteristics of cytotoxicity and anti-metastasis has generated intense interest among the invasive cancer study. Curcumin, one of these anti-cancer phytochemicals, has been reported to induce the cytoprotective enzyme heme oxygenase-1 expression. Since heme oxygenase-1 has been suggested to enhance cancer cell invasion, we investigated the anti-invasive effect of curcumin when heme oxygenase-1 was knocked down in vitro, and the heme oxygenase-1 expression after curcumin treatment in vivo. Curcumin inhibited cell viability and the MMP-2/9 activities of human bladder cancer cells. At 10μM, curcumin inhibited cell viability and cell invasive activity by 15{\%} and 40{\%}, respectively. Ten micrometer curcumin increased the intracellular reactive oxygen species concentration and heme oxygenase-1 protein and mRNA expression in bladder cancer cells. The anti-invasive activity of curcumin was elevated when heme oxygenase-1 was knocked down by siRNA or inhibited by pharmacological inhibitor. In vivo, curcumin induced heme oxygenase-1 protein expression in the lung tissue of murine lung metastasis tumor model and in the bladder tissue of murine orthotopic bladder tumor model. Taken together, our data suggest that curcumin-induced heme oxygenase-1 attenuates the anti-invasive effect of curcumin in cancer therapy, and co-treatment by heme oxygenase-1 inhibitor enhances the anti-invasive activity of curcumin.",
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AB - Some phytochemicals with the characteristics of cytotoxicity and anti-metastasis has generated intense interest among the invasive cancer study. Curcumin, one of these anti-cancer phytochemicals, has been reported to induce the cytoprotective enzyme heme oxygenase-1 expression. Since heme oxygenase-1 has been suggested to enhance cancer cell invasion, we investigated the anti-invasive effect of curcumin when heme oxygenase-1 was knocked down in vitro, and the heme oxygenase-1 expression after curcumin treatment in vivo. Curcumin inhibited cell viability and the MMP-2/9 activities of human bladder cancer cells. At 10μM, curcumin inhibited cell viability and cell invasive activity by 15% and 40%, respectively. Ten micrometer curcumin increased the intracellular reactive oxygen species concentration and heme oxygenase-1 protein and mRNA expression in bladder cancer cells. The anti-invasive activity of curcumin was elevated when heme oxygenase-1 was knocked down by siRNA or inhibited by pharmacological inhibitor. In vivo, curcumin induced heme oxygenase-1 protein expression in the lung tissue of murine lung metastasis tumor model and in the bladder tissue of murine orthotopic bladder tumor model. Taken together, our data suggest that curcumin-induced heme oxygenase-1 attenuates the anti-invasive effect of curcumin in cancer therapy, and co-treatment by heme oxygenase-1 inhibitor enhances the anti-invasive activity of curcumin.

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