Cuneothalamic relay neurons are postsynaptic to glycine-immunoreactive terminals in the rat cuneate nucleus

June Horng Lue, Jeng Yung Shieh, Chen Yuan Wen, Seu Hwa Chen

研究成果: 雜誌貢獻文章同行評審

9 引文 斯高帕斯(Scopus)

摘要

This study was aimed to clarify whether the cuneothalamic relay neurons (CTNs) in the rat cuneate nucleus contained glycine or whether the neurons were modulated directly by presynaptic glycine-IR terminals. For this purpose, retrograde transport of wheat germ agglutinin conjugated with horseradish peroxidase (WGA-HRP) and immunoperoxidase labelling for glycine have been used to ascertain if the CTNs in the rat are glycine-immunoreactive (glycine-IR). Our results have shown that the WGA-HRP-labelled CTNs (mean area = 318 ± 6.5 μm2) were not reactive for glycine. Glycine immunoreactivity, however, was localized in some small-sized neurons (mean area = 210 ± 6.2 μm2) and axon terminals associated with the CTNs. The synaptic organization between the glycine-IR terminals and CTNs was further analyzed using anti-glycine postembedding immunogold labelling. By electron microscopy, the immunogold-labelled glycine-IR terminals containing pleomorphic synaptic vesicles formed symmetrical synaptic contacts with the dendrites, dendritic spines, and somata of CTNs. Quantitative estimation showed that the mean ratios of glycine-IR terminals to total terminals associated with the soma, proximal dendrites and distal dendrites of the CTN were 49.5, 45.2, and 45.8%, respectively. The higher incidence of glycine-IR terminals on the soma, however, was not significantly different from that of the proximal and distal dendrites. Notwithstanding the above, this study has shown a large number of glycine-IR terminals making direct synaptic contacts with CTNs, suggesting that glycine is one of the important neurotransmitters involved in postsynaptic inhibition on the cuneothalamic relay neurons to modulate incoming somatosensory information from forelimb areas in the rat. (C) 2000 Wiley-Liss, Inc.

原文英語
頁(從 - 到)222-231
頁數10
期刊Synapse
37
發行號3
DOIs
出版狀態已發佈 - 九月 1 2000
對外發佈

ASJC Scopus subject areas

  • 神經科學 (全部)
  • 生理學
  • 藥理

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