Crystallization of oligonucleotides and their complexes with antitumor drugs

Andrew H.J. Wang, Yi Gui Gao

研究成果: 雜誌貢獻文章同行評審

25 引文 斯高帕斯(Scopus)

摘要

Single-crystal analysis of DNA and RNA oligonucleotides and their complexes with drugs has provided a great deal of information concerning the structure and function of these molecules. Its success depends critically on the probability of obtaining suitable crystals, which so far appears to be not very high. In this paper we present a detailed view of the crystallization process and several representative examples of the crystallization of oligonucleotides. Special emphases are placed on two aspects, the materials and the techniques. The preparation, purity, and selection of sequences of oligonucleotides are discussed. Some important factors, such as metal ions, organic ions, pH, precipitants, and various additives, are evaluated. Two specific examples of crystallizing Z-DNA and the complexes between DNA and the antitumor drug nogalamycin are presented. Special techniques such as feeding, seeding, and crystal recycling are also described.

原文英語
頁(從 - 到)91-99
頁數9
期刊Methods
1
發行號1
DOIs
出版狀態已發佈 - 8月 1990
對外發佈

ASJC Scopus subject areas

  • 分子生物學
  • 生物化學、遺傳與分子生物學 (全部)

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