Crystallization and preliminary X-ray diffraction characterization of RpfF, a key DSF synthase from Stenotrophomonas maltophilia

Xian Ya Lin, Shu Ju Liao, Ko Hsin Chin, Hui Ling Shih, Gwan Han Shen, Andrew H.J. Wang, Shan Ho Chou

研究成果: 雜誌貢獻文章同行評審

摘要

Stenotrophomonas maltophilia has emerged as a critical nosocomial opportunistic pathogen in the last few years. It is resistant to many clinically useful antibiotics; hence, new ways of combatting this bacterium are essential. Diffusible signal factor (DSF) dependent quorum sensing is a major mechanism of virulence induction in S. maltophilia, with RpfF playing a key role in DSF biosynthesis. Inhibiting S. maltophilia RpfF (SmRpfF) function via small-molecule interference may constitute a new way of treating S. maltophilia infection. SmRpfF was therefore overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to the tetragonal space group P41212 or P43212, with unit-cell parameters a = b = 148.51, c = 122.82 Å, and diffracted to a resolution of 2.25 Å.

原文英語
頁(從 - 到)1021-1023
頁數3
期刊Acta Crystallographica Section F: Structural Biology and Crystallization Communications
65
發行號10
DOIs
出版狀態已發佈 - 2009
對外發佈

ASJC Scopus subject areas

  • 生物物理學
  • 結構生物學
  • 生物化學
  • 遺傳學
  • 凝聚態物理學

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