Crystal structures of the chromosomal proteins Sso7d/Sac7d bound to DNA containing T-G mismatched base-pairs

Shaoyu Su, Yi Gui Gao, Howard Robinson, Yen Chywan Liaw, Stephen P. Edmondson, John W. Shriver, Andrew H.J. Wang

研究成果: 雜誌貢獻文章同行評審

37 引文 斯高帕斯(Scopus)

摘要

Sso7d and Sac7d are two small chromatin proteins from the hyperthermophilic archaeabacterium Sulfolobus solfataricus and Sulfolobus acidocaldarius, respectively. The crystal structures of Sso7d-GTGATCGC, Sac7d-GTGATCGC and Sac7d-GTGATCAC have been determined and refined at 1.45 Å, 2.2 Å and 2.2 Å, respectively, to investigate the DNA binding property of Sso7d/Sac7d in the presence of a T-G mismatch base-pair. Detailed structural analysis revealed that the intercalation site includes the T-G mismatch base-pair and Sso7d/Sac7d bind to that mismatch base-pair in a manner similar to regular DNA. In the Sso7d-GTGATCGC complex, a new inter-strand hydrogen bond between T2O4 and C14N4 is formed and well-order bridging water molecules are found. The results suggest that the less stable DNA stacking site involving a T-G mismatch may be a preferred site for protein side-chain intercalation. (C) 2000 Academic Press.

原文英語
頁(從 - 到)395-403
頁數9
期刊Journal of Molecular Biology
303
發行號3
DOIs
出版狀態已發佈 - 10月 27 2000
對外發佈

ASJC Scopus subject areas

  • 生物物理學
  • 結構生物學
  • 分子生物學

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