Crystal structure of IcaR, a repressor of the TetR family implicated in biofilm formation in staphylococcus epidermidis

Wen Yih Jeng, Tzu Ping Ko, Chia I. Liu, Rey Ting Guo, Chien Liang Liu, Hui Lin Shr, Andrew H J Wang

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45 引文 斯高帕斯(Scopus)

摘要

Expression of the gene cluster icaADBC is necessary for biofilm production in Staphylococcus epidermidis. The ica operon is negatively controlled by the repressor IcaR. Here, the crystal structure of IcaR was determined and the refined structure revealed a homodimer comprising entirely α-helices, typical of the tetracycline repressor protein family for gene regulations. The N-terminal domain contains a conserved helix-turn-helix DNA-binding motif with some conformational variations, indicating flexibility in this region. The C-terminal domain shows a complementary surface charge distribution about the dyad axis, ideal for efficient and specific dimer formation. The results of the electrophoretic mobility shift assay and isothermal titration calorimetry suggested that a 28 bp core segment of the ica operator is implicated in the cooperative binding of two IcaR dimers on opposite sides of the duplex DNA. Computer modeling based on the known DNA-complex structure of QacR and site-specific mutagenesis experiments showed that direct protein-DNA interactions are mostly conserved, but with slight variations for recognizing the different sequences. By interfering with the binding of IcaR to DNA, aminoglycoside gentamicin and other antibiotics may activate the icaADBC genes and elicit biofilm production in S. epidermidis, and likely S. aureus, as a defense mechanism.
原文英語
頁(從 - 到)1567-1577
頁數11
期刊Nucleic Acids Research
36
發行號5
DOIs
出版狀態已發佈 - 三月 2008
對外發佈

ASJC Scopus subject areas

  • 遺傳學

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