Critical cysteine residues for regulation of integrin αIIbβ3 are clustered in the epidermal growth factor domains of the β3 subunit

Tetsuji Kamata, Hironobu Ambo, Wilma Puzon-McLaughlin, Kenneth Khiem Tieu, Makoto Handa, Yasuo Ikeda, Yoshikazu Takada

研究成果: 雜誌貢獻文章

52 引文 (Scopus)

摘要

Chemical or enzymic reduction/oxidation of integrin cysteine residues (e.g. by reducing agents and protein disulphide isomerase) may be a mechanism for regulating integrin function. It has also been proposed that unique cysteine residues in the integrin β3 subunit are involved in the regulation of αIIbβ3. In the present study, we studied systematically the role of disulphide bonds in β3 on the ligand-binding function of αIIbβ3 by mutating individual cysteine residues of β3 to serine. We found that the disulphide bonds that are critical for αIIbβ3 regulation are clustered within the EGF (epidermal growth factor) domains. Interestingly, disrupting only a single disulphide bond in the EGF domains was enough to activate αIIbβ3 fully. In contrast, only two (of 13) disulphide bonds tested outside the EGF domains activated αIIbβ3. These results suggest that the disulphide bonds in the EGF domains should be intact to keep αIIbβ3 in an inactive state, and that there is no unique cysteine residue in the EGF domain critical for regulating the receptor. The cysteine residues in the EGF domains are potential targets for chemical or enzymic reduction.

原文英語
頁(從 - 到)1079-1082
頁數4
期刊Biochemical Journal
378
發行號3
DOIs
出版狀態已發佈 - 三月 15 2004
對外發佈Yes

指紋

Epidermal Growth Factor
Integrins
Cysteine
Disulfides
Protein Disulfide-Isomerases
Reducing Agents
Serine
Ligands
Oxidation

ASJC Scopus subject areas

  • Biochemistry

引用此文

Critical cysteine residues for regulation of integrin αIIbβ3 are clustered in the epidermal growth factor domains of the β3 subunit. / Kamata, Tetsuji; Ambo, Hironobu; Puzon-McLaughlin, Wilma; Tieu, Kenneth Khiem; Handa, Makoto; Ikeda, Yasuo; Takada, Yoshikazu.

於: Biochemical Journal, 卷 378, 編號 3, 15.03.2004, p. 1079-1082.

研究成果: 雜誌貢獻文章

Kamata, T, Ambo, H, Puzon-McLaughlin, W, Tieu, KK, Handa, M, Ikeda, Y & Takada, Y 2004, 'Critical cysteine residues for regulation of integrin αIIbβ3 are clustered in the epidermal growth factor domains of the β3 subunit', Biochemical Journal, 卷 378, 編號 3, 頁 1079-1082. https://doi.org/10.1042/BJ20031701
Kamata, Tetsuji ; Ambo, Hironobu ; Puzon-McLaughlin, Wilma ; Tieu, Kenneth Khiem ; Handa, Makoto ; Ikeda, Yasuo ; Takada, Yoshikazu. / Critical cysteine residues for regulation of integrin αIIbβ3 are clustered in the epidermal growth factor domains of the β3 subunit. 於: Biochemical Journal. 2004 ; 卷 378, 編號 3. 頁 1079-1082.
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