Correlation of expression of CD44 isoforms and E-cadherin with differentiation in human urothelial cell lines and transitional cell carcinoma

Ruey Long Hong, Yeong Shiau Pu, Jan Show Chu, Wei Jei Lee, Yao Chang Chen, Cheng Wen Wu

研究成果: 雜誌貢獻文章

32 引文 (Scopus)

摘要

Using immunostaining, immunoblot, reverse-transcriptase polymerase chain reaction and Southern blot, we found that expressions of CD44 isoforms and E-cadherin were very closely linked and were correlated with the differentiation status in human urothelial cell lines and clinical specimens of transitional cell carcinoma. Normal urothelium, well to moderately differentiated cell lines and surgical samples expressed E-cadherin and large CD44 isoforms containing exon v6, which was pivotal in metastasis of rat pancreatic cell line model. Poorly differentiated cell lines and surgical samples, were E-cadherin-negative and expressed primarily standard form CD44, which did not contain exon v6. We concluded that CD44v6 isoforms and E-cadherin were both down-regulated during the carcinogenesis of urothelium. The large exon v6 containing CD44 isoforms were readily detected in normal urothelium, therefore, were not likely linked to cancer metastasis. E-cadherin and CD44v6 may be used as differentiation markers for human urothelial tumors. Immunohistochemical study solely with antibody against epitopes encoded by exon v6 alone is not informative enough as other alternatively spliced exons may change the function of CD44v6 isoforms.

原文英語
頁(從 - 到)81-87
頁數7
期刊Cancer Letters
89
發行號1
DOIs
出版狀態已發佈 - 二月 10 1995
對外發佈Yes

指紋

Transitional Cell Carcinoma
Cadherins
Exons
Protein Isoforms
Urothelium
Cell Line
Neoplasm Metastasis
Differentiation Antigens
Southern Blotting
Reverse Transcriptase Polymerase Chain Reaction
Epitopes
Neoplasms
Carcinogenesis
Antibodies

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Molecular Biology

引用此文

Correlation of expression of CD44 isoforms and E-cadherin with differentiation in human urothelial cell lines and transitional cell carcinoma. / Hong, Ruey Long; Pu, Yeong Shiau; Chu, Jan Show; Lee, Wei Jei; Chen, Yao Chang; Wu, Cheng Wen.

於: Cancer Letters, 卷 89, 編號 1, 10.02.1995, p. 81-87.

研究成果: 雜誌貢獻文章

Hong, Ruey Long ; Pu, Yeong Shiau ; Chu, Jan Show ; Lee, Wei Jei ; Chen, Yao Chang ; Wu, Cheng Wen. / Correlation of expression of CD44 isoforms and E-cadherin with differentiation in human urothelial cell lines and transitional cell carcinoma. 於: Cancer Letters. 1995 ; 卷 89, 編號 1. 頁 81-87.
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AU - Pu, Yeong Shiau

AU - Chu, Jan Show

AU - Lee, Wei Jei

AU - Chen, Yao Chang

AU - Wu, Cheng Wen

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N2 - Using immunostaining, immunoblot, reverse-transcriptase polymerase chain reaction and Southern blot, we found that expressions of CD44 isoforms and E-cadherin were very closely linked and were correlated with the differentiation status in human urothelial cell lines and clinical specimens of transitional cell carcinoma. Normal urothelium, well to moderately differentiated cell lines and surgical samples expressed E-cadherin and large CD44 isoforms containing exon v6, which was pivotal in metastasis of rat pancreatic cell line model. Poorly differentiated cell lines and surgical samples, were E-cadherin-negative and expressed primarily standard form CD44, which did not contain exon v6. We concluded that CD44v6 isoforms and E-cadherin were both down-regulated during the carcinogenesis of urothelium. The large exon v6 containing CD44 isoforms were readily detected in normal urothelium, therefore, were not likely linked to cancer metastasis. E-cadherin and CD44v6 may be used as differentiation markers for human urothelial tumors. Immunohistochemical study solely with antibody against epitopes encoded by exon v6 alone is not informative enough as other alternatively spliced exons may change the function of CD44v6 isoforms.

AB - Using immunostaining, immunoblot, reverse-transcriptase polymerase chain reaction and Southern blot, we found that expressions of CD44 isoforms and E-cadherin were very closely linked and were correlated with the differentiation status in human urothelial cell lines and clinical specimens of transitional cell carcinoma. Normal urothelium, well to moderately differentiated cell lines and surgical samples expressed E-cadherin and large CD44 isoforms containing exon v6, which was pivotal in metastasis of rat pancreatic cell line model. Poorly differentiated cell lines and surgical samples, were E-cadherin-negative and expressed primarily standard form CD44, which did not contain exon v6. We concluded that CD44v6 isoforms and E-cadherin were both down-regulated during the carcinogenesis of urothelium. The large exon v6 containing CD44 isoforms were readily detected in normal urothelium, therefore, were not likely linked to cancer metastasis. E-cadherin and CD44v6 may be used as differentiation markers for human urothelial tumors. Immunohistochemical study solely with antibody against epitopes encoded by exon v6 alone is not informative enough as other alternatively spliced exons may change the function of CD44v6 isoforms.

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