摘要
We examined the expression, abundance, and protein kinase activity of pp60(c-src) in two different pairs of genetically indistinguishable cloned human neuroblastoma cell variants which display altered phenotypes as the result of conversion from a neuronal to a non-neuronal phenotype. The results demonstrate that cells which exhibit the neuroblastic (N-type) phenotype possess high levels of pp60(c-src) protein and that one of the two N-type cell lines is capable of expressing the neuronal-specific isoenzyme of pp60(c-src). In contrast, cells which display the substrate-adherent (S-type) phenotype have low levels of pp60(c-src) protein and express exclusively the non-neuronal isoenzyme of pp60(c-src). In all cells examined the abundance of pp60(c-src) was found to be proportional to the steady-state level of c-src RNA. In each case the protein kinase activity of pp60(c-src) was found to be proportional to the abundance of the protein and independent of the ratio of neuronal to non-neuronal species of pp60(c-src).
原文 | 英語 |
---|---|
頁(從 - 到) | 421-427 |
頁數 | 7 |
期刊 | Oncogene |
卷 | 4 |
發行號 | 4 |
出版狀態 | 已發佈 - 1月 1 1989 |
對外發佈 | 是 |
ASJC Scopus subject areas
- 分子生物學
- 遺傳學
- 癌症研究