Coordinate alteration of pp60(c-src) abundance and c-src RNA expression in human neuroblastoma variants

A. Veillette, J. O'Shaughnessy, I. D. Horak, M. A. Israel, D. Yee, N. Rosen, D. J. Fujita, H. J. Kung, J. L. Biedler, J. B. Bolen

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12 引文 斯高帕斯(Scopus)

摘要

We examined the expression, abundance, and protein kinase activity of pp60(c-src) in two different pairs of genetically indistinguishable cloned human neuroblastoma cell variants which display altered phenotypes as the result of conversion from a neuronal to a non-neuronal phenotype. The results demonstrate that cells which exhibit the neuroblastic (N-type) phenotype possess high levels of pp60(c-src) protein and that one of the two N-type cell lines is capable of expressing the neuronal-specific isoenzyme of pp60(c-src). In contrast, cells which display the substrate-adherent (S-type) phenotype have low levels of pp60(c-src) protein and express exclusively the non-neuronal isoenzyme of pp60(c-src). In all cells examined the abundance of pp60(c-src) was found to be proportional to the steady-state level of c-src RNA. In each case the protein kinase activity of pp60(c-src) was found to be proportional to the abundance of the protein and independent of the ratio of neuronal to non-neuronal species of pp60(c-src).
原文英語
頁(從 - 到)421-427
頁數7
期刊Oncogene
4
發行號4
出版狀態已發佈 - 一月 1 1989
對外發佈

ASJC Scopus subject areas

  • 分子生物學
  • 遺傳學
  • 癌症研究

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