Controlled release of BMP-2 by chitosan/γ-PGA polyelectrolyte multilayers coating on titanium alloy promotes osteogenic differentiation in rat bone-marrow mesenchymal stem cells

Ren Jei Chung, Keng Liang Ou, Wei Kai Tseng, Hsuan Liang Liu

研究成果: 雜誌貢獻文章同行評審

13 引文 斯高帕斯(Scopus)

摘要

The excellent biocompatibility and mechanical properties of titanium alloys have led to their wide-scale application in orthopedic implants. Appropriate surface treatment of these alloys can greatly improve cellular attachment as well as interactions between implants and tissue. This paper reports on the preparation of polyelectrolyte multilayers (PEM) coating comprising chitosan and gamma-poly(glutamic acid) on a Ti6Al4V substrate via spin-coating. Bone morphogenetic protein 2 was then loaded directly into the coating. Following sample preparation, we performed in vitro studies using SD rat bone-marrow mesenchymal stem cells (rBMSCs) to investigate the biological effects of the coating. Our results demonstrate that this chitosan/γ-PGA PEM coating is highly stable and does not have any adverse effects on rBMSCs. The proposed coating proved highly effective in carrying and enabling the gradual release of BMP-2. At day 14, the coated samples presented osteogenic differentiation far exceeding that of the non-coated samples. After co-culturing for 21. days, the calcium content in the PEM coating group was double that of the non-coated group, which is a clear indication of enhanced cellular mineralization.
原文英語
頁(從 - 到)283-288
頁數6
期刊Surface and Coatings Technology
303
DOIs
出版狀態已發佈 - 十月 15 2016

ASJC Scopus subject areas

  • 化學 (全部)
  • 凝聚態物理學
  • 材料化學
  • 表面、塗料和薄膜
  • 表面和介面

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