Constituents of aquilaria sinensis leaves upregulate the expression of matrix metalloproteases 2 and 9

Sui Wen Hsiao, Yu Chin Wu, Hui Ching Mei, Yu Hsin Chen, George Hsiao, Ching Kuo Lee

研究成果: 雜誌貢獻文章同行評審

摘要

In this novel study, we isolated 28 compounds from the leaves of Aquilaria sinensis (Lour.) Gilg based on a bioassay-guided procedure and also discovered the possible matrix metalloprotease 2 (MMP-2) and 9 (MMP-9) modulatory effect of pheophorbide A (PA). To evaluate the regulatory activity on MMP-2 and MMP-9, the HT-1080 human fibrosarcoma cells were treated with various concentrations of extracted materials and isolated compounds. PA was extracted by methanol from the leaves of A. sinensis and separated from the fraction of the partitioned ethyl acetate layer. PA is believed to be an active component for MMP expression since it exhibited significant stimulation on MMP-2 and proMMP-9 activity. When treating with 50 µM of PA, the expression of MMP-2 and MMP-9 were increased 1.9-fold and 2.3-fold, respectively. PA also exhibited no cytotoxicity against HT-1080 cells when the cell viability was monitored. Furthermore, no significant MMP activity was observed when five PA analogues were evaluated. This study is the first to demonstrate that C-17 of PA is the deciding factor in determining the bioactivity of the compound. The MMP-2 and proMMP-9 modulatory activity of PA indicate its potential applications for reducing scar formation and comparative medical purposes.

原文英語
文章編號2537
期刊Molecules
26
發行號9
DOIs
出版狀態已發佈 - 2021

ASJC Scopus subject areas

  • 分析化學
  • 化學(雜項)
  • 分子醫學
  • 藥學科學
  • 藥物發現
  • 物理與理論化學
  • 有機化學

指紋

深入研究「Constituents of aquilaria sinensis leaves upregulate the expression of matrix metalloproteases 2 and 9」主題。共同形成了獨特的指紋。

引用此