Confocal laser scanning microscopy: I. An overview of principle and practice in biomedical research

Jui Tai Chen, Ruei-Ming Chen, Yi Ling Lin, Huai-Chia Chang, Yu Hua Lin, Ta-Liang Chen, Tyng-Guey Chen

研究成果: 雜誌貢獻文章同行評審

13 引文 斯高帕斯(Scopus)

摘要

Evolving from conventional microscopic technologies, confocal microscopy has proved itself to play an important role in the biomedical research during the past decade. Confocal microscope has many advantages over traditional microscope including the ability to look deeply into inside cells with less photodamage and photobleach, reconstruct three-dimensional images, and chart intracellular dynamic events in the living cells. With these remarkable properties and the availability of fluorescent dyes for living cells, the confocal microscopy has been widely used in solving many unknown questions in biological and pharmacological fields. In clinics, confocal microscope has also served as an important tool to observe the living cells in skins and eyes. For anesthesiologists, confocal microscope has made possible novel experimental approaches for the effects of multiple anesthetic agents on cells. Furthermore, the technology of fibreoptical confocal endomicroscopy is now on its way of maturation. It will soon be the era for confocal microscopy to explore the "cell behavior" inside of intact living tissues.
原文英語
頁(從 - 到)33-40
頁數8
期刊Acta Anaesthesiologica Taiwanica
42
發行號1
出版狀態已發佈 - 三月 2004

ASJC Scopus subject areas

  • 麻醉與疼痛醫學

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