Computational identification of riboswitches based on RNA conserved functional sequences and conformations

Tzu H. Chang, Hsien D. Huang, Li Ching Wu, Chi T. Yeh, Baw Jhiune Liu, Jorng Tzong Horng

研究成果: 雜誌貢獻文章

42 引文 斯高帕斯(Scopus)

摘要

Riboswitches are cis-acting genetic regulatory elements within a specific mRNA that can regulate both transcription and translation by interacting with their corresponding metabolites. Recently, an increasing number of riboswitches have been identified in different species and investigated for their roles in regulatory functions. Both the sequence contexts and structural conformations are important characteristics of riboswitches. None of the previously developed tools, such as covariance models (CMs), Riboswitch finder, and RibEx, provide a web server for efficiently searching homologous instances of known riboswitches or considers two crucial characteristics of each riboswitch, such as the structural conformations and sequence contexts of functional regions. Therefore, we developed a systematic method for identifying 12 kinds of riboswitches. The method is implemented and provided as a web server, RiboSW, to efficiently and conveniently identify riboswitches within messenger RNA sequences. The predictive accuracy of the proposed method is comparable with other previous tools. The efficiency of the proposed method for identifying riboswitches was improved in order to achieve a reasonable computational time required for the prediction, which makes it possible to have an accurate and convenient web server for biologists to obtain the results of their analysis of a given mRNA sequence. RiboSW is now available on the web at http://RiboSW.mbc.nctu.edu.tw/. Published by Cold Spring Harbor Laboratory Press.

原文英語
頁(從 - 到)1426-1430
頁數5
期刊RNA
15
發行號7
DOIs
出版狀態已發佈 - 七月 2009
對外發佈Yes

ASJC Scopus subject areas

  • Molecular Biology

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