摘要

Triflavin, an Arg-Gly-Asp (RGD)-containing disintegrin purified from venom peptide inhibited platelet aggregation by interfering with the interaction of fibrinogen with αIIbβ3 integrin. Using an immunostaining technique and electron microscopy, we investigated and compared the distribution of triflavin binding in both resting and activated platelets. Triflavin uniformly and strongly stained the plasma membrane and the open canalicular system (OCS), whereas a lesser extent of staining was seen on α-granules in both resting and activated platelets. Furthermore, resting unfixed platelets were incubated with triflavin for 10 min at 4°C, and then rewarmed at 30°C for 0, 10, and 30 min to advance internalization. At 0 min, platelets showed an extensive rim-staining pattern of bound triflavin on the surface membrane, which was then gradually internalized into the cytoplasmic OCS with prolonging of incubation times. However, triflavin bound fewer to α-granules than to the OCS within the 0-30-min period of internalization in both resting and activated platelets. Furthermore, triflavin did not influence physiological endocytosis in resting platelets. Comparing the 3D structures of triflavin and another disintegrin, echistatin, we found that the spatial differences between the RGD motif and the C-termini of structures of disintegrins may mediate functional differences of binding activity towards αIIbβ3 integrin in resting platelets. These data indicate that (1) triflavin binds effectively to αIIbβ3 on the platelet membrane and cytoplasmic OCS, but a relative lesser extent to α-granules in both resting and activated platelets; (2) triflavin is internalized in resting platelets independent of cellular activation; and (3) spatial differences between the RGD motif and the C-termini of disintegrins may play an important role in mediating disintegrin binding to αIIbβ3 in resting platelets.
原文英語
頁(從 - 到)37-46
頁數10
期刊Thrombosis Research
109
發行號1
DOIs
出版狀態已發佈 - 一月 1 2003

ASJC Scopus subject areas

  • 心臟病學與心血管醫學
  • 血液學

指紋

深入研究「Comparison of the binding character of triflavin on resting and activated α<sub>IIb</sub>β<sub>3</sub> integrin in human platelets by electron microscopy」主題。共同形成了獨特的指紋。

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