Comparison of Quinn's Advantage fertilization medium and tissue culture medium 199 for invitro maturation of oocytes

Yu Hung Lin, Jiann-Loung Hwang, Lee Wen Huang, Kok Min Seow, Bih Chwen Hsieh, Chii Ruey Tzeng

研究成果: 雜誌貢獻文章同行評審

2 引文 斯高帕斯(Scopus)

摘要

Objective: The purpose of the study was to compare the Quinn's Advantage fertilization medium (Q1) and the tissue culture medium 199 (TCM199) for invitro maturation (IVM) of oocytes and ammonium production during IVM. Materials and methods: The immature murine oocytes were randomly added into Q1 and TCM199. Ammonium concentrations were measured at the start and after 18hours of IVM, and the mature oocytes were fertilized and cultured into blastocysts. The blastocysts were then stained for inner cell mass (ICM) and trophectoderm. Results: The maturation rate was higher in Q1 than in TCM199 (85.7% vs. 76.6%, p=0.024). The fertilization and blastocyst rates were slightly higher in Q1, but not significant. Differential staining of the blastocysts showed slightly higher ICM ratio in the blastocysts derived from Q1. Mean ammonium concentrations in Q1 and TCM199 at Time 0 were 184.9 and 339.2μg/dL, respectively (p=0.05), and after 18hours of IVM were 268.7 and 443.6μg/dL, respectively (p=0.045). Addition of ammonium chloride into Q1 adversely affects IVM. Conclusion: Q1 is superior to TCM199 in terms of oocyte maturation, which may be due to lower ammonium concentration.
原文英語
頁(從 - 到)17-20
頁數4
期刊Taiwanese Journal of Obstetrics and Gynecology
53
發行號1
DOIs
出版狀態已發佈 - 三月 2014

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

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