Comparison of Next‐Generation Sequencing and Polymerase Chain Reaction for Personalized Treatment‐Related Genomic Status in Patients with Metastatic Colorectal Cancer

Wei Chih Su, Yi Chen Tsai, Hsiang Lin Tsai, Tsung Kun Chang, Tzu Chieh Yin, Ching Wen Huang, Yen Cheng Chen, Ching Chun Li, Po Jung Chen, Yun Ru Liu, Tsung Han Hsieh, Jaw Yuan Wang

研究成果: 雜誌貢獻文章同行評審

摘要

Personalized treatments based on the genetic profiles of tumors can simultaneously optimize efficacy and minimize toxicity, which is beneficial for improving patient outcomes. This study aimed to integrate gene alterations associated with predictive and prognostic outcomes in patients with metastatic colorectal cancer (mCRC) with polymerase chain reaction (PCR) and in‐house nextgeneration sequencing (NGS) to detect KRAS, NRAS, and BRAF mutations. In the present study, 41 patients with mCRC were assessed between August 2017 and June 2019 at a single institution. The overall concordance between NGS and PCR results for detecting KRAS, NRAS, and BRAF mutations was considerably high (87.8–92.7%), with only 15 discrepant results between PCR and NGS. Our companion diagnostic test analyzes KRAS, NRAS, and BRAF as a panel of CRC molecular targets; therefore, it has the advantages of requiring fewer specimens and being more time and cost efficient than conventional testing for separate analyses, allowing for the simultaneous analysis of multiple genes.

原文英語
文章編號1563
期刊Current Issues in Molecular Biology
44
發行號4
DOIs
出版狀態已發佈 - 4月 2022

ASJC Scopus subject areas

  • 微生物學
  • 分子生物學
  • 微生物學(醫學)

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