Comparison of in vitro proliferative capacity of human periodontal ligament cells in juvenile and aged donors

F. Nishimura, V. P. Terranova, M. Braithwaite, R. Orman, H. Ohyama, J. Mineshiba, H. H. Chou, S. Takashiba, Y. Murayama

研究成果: 雜誌貢獻文章同行評審

24 引文 斯高帕斯(Scopus)

摘要

OBJECTIVE: The aim of this study is to compare the in vitro proliferative capacity of periodontal ligament (PDL) cells from aged and juvenile donors. MATERIALS AND METHODS: Flow-cytometric analysis of the cell cycle was used to compare the length of each cell cycle, and the ratio of the cells progressing through the cycles between four PDL cells from juvenile donors and four cells from aged donors. Then, replicative capacity of the PDL cells from three juvenile and three aged donors was compared by serial cultures. Finally, expression of c-fos was compared between cells proliferating and cells which had reached senescent. RESULTS: Flow- cytometric analysis of the cell cycle had revealed that although there were no differences in the length of each phase of the cell cycle, significant differences were found in the ratio of the cells entering from Gap I to DNA synthesis phase of the cell cycle (P <0.025). Replicative capacity was much longer in two cells from juvenile donors (about 20 population doublings), while all cells from aged donors showed short dividing abilities (less than eight population doublings), hence entered senescent phases shortly. Additionally, no c-fos was detected in cells which had reached senescence upon stimulation with serum. CONCLUSIONS: It is generally believed that aged humans have an impaired wound healing ability. We believe that more fibrotic PDL tissues seen in aged humans might be the reason for this, and suggest that this phenomena might be due to the progressive accumulation of senescent cell populations.
原文英語
頁(從 - 到)162-166
頁數5
期刊Oral Diseases
3
發行號3
出版狀態已發佈 - 9月 1997
對外發佈

ASJC Scopus subject areas

  • 牙科 (全部)

指紋

深入研究「Comparison of in vitro proliferative capacity of human periodontal ligament cells in juvenile and aged donors」主題。共同形成了獨特的指紋。

引用此