Comparison of glutathione peroxidase-3 protein expression and enzyme bioactivity in normal subjects and patients with sepsis

Wei Ju Lee, Yen Ling Chen, Yi Wen Chu, Du Shieng Chien

研究成果: 雜誌貢獻文章

摘要

Background: Serum glutathione peroxidase-3 (GPx-3) is known as a key selenoprotein with antioxidant properties. GPx-3 deficiency has been associated with sepsis. The objectives of this study are (1) to compare the GPx-3 protein concentrations and GPx-3 bioactivity in normal healthy subjects and septic patients, and (2) to evaluate the relationship between GPx-3 bioactivity and its protein concentration. Methods: Serum samples were collected from 50 normal healthy subjects and 70 septic patients. The reliable bioanalytical methods for GPx-3 protein concentration and bioactivity in human serum were developed and validated. Analyses of GPx-3 bioactivity and GPx-3 protein concentration were then performed. Results: Geometric mean GPx-3 bioactivity was 78.13. U/l for patients with sepsis, significantly lower than normal subjects with 108.21. U/l (p <. 0.0001). Similarly, the GPx-3 protein concentration was significantly lower in patients with sepsis than in normal subjects, with the mean GPx-3 value of 0.78 vs 3.10. μg/ml, respectively (p <. 0.0001). A positive correlation was observed between the GPx-3 bioactivity and its corresponding protein concentration in septic serum samples (R = 0.74, p <. 0.0001), regardless of gender or age difference. Conclusion: These findings suggest that the decrease in GPx-3 bioactivity observed in the septic patients was resulted from the significant sepsis-related decline of GPx-3 protein concentrations.
原文英語
期刊Clinica Chimica Acta
DOIs
出版狀態已發佈 - 2019
對外發佈Yes

指紋

Glutathione Peroxidase
Bioactivity
Sepsis
Enzymes
Proteins
Serum
Healthy Volunteers
Selenoproteins
Antioxidants

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

引用此文

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title = "Comparison of glutathione peroxidase-3 protein expression and enzyme bioactivity in normal subjects and patients with sepsis",
abstract = "Background: Serum glutathione peroxidase-3 (GPx-3) is known as a key selenoprotein with antioxidant properties. GPx-3 deficiency has been associated with sepsis. The objectives of this study are (1) to compare the GPx-3 protein concentrations and GPx-3 bioactivity in normal healthy subjects and septic patients, and (2) to evaluate the relationship between GPx-3 bioactivity and its protein concentration. Methods: Serum samples were collected from 50 normal healthy subjects and 70 septic patients. The reliable bioanalytical methods for GPx-3 protein concentration and bioactivity in human serum were developed and validated. Analyses of GPx-3 bioactivity and GPx-3 protein concentration were then performed. Results: Geometric mean GPx-3 bioactivity was 78.13. U/l for patients with sepsis, significantly lower than normal subjects with 108.21. U/l (p <. 0.0001). Similarly, the GPx-3 protein concentration was significantly lower in patients with sepsis than in normal subjects, with the mean GPx-3 value of 0.78 vs 3.10. μg/ml, respectively (p <. 0.0001). A positive correlation was observed between the GPx-3 bioactivity and its corresponding protein concentration in septic serum samples (R = 0.74, p <. 0.0001), regardless of gender or age difference. Conclusion: These findings suggest that the decrease in GPx-3 bioactivity observed in the septic patients was resulted from the significant sepsis-related decline of GPx-3 protein concentrations.",
keywords = "Biomarker, GPx-3 bioactivity, GPx-3 protein concentration, Selenium, Sepsis",
author = "Lee, {Wei Ju} and Chen, {Yen Ling} and Chu, {Yi Wen} and Chien, {Du Shieng}",
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T1 - Comparison of glutathione peroxidase-3 protein expression and enzyme bioactivity in normal subjects and patients with sepsis

AU - Lee, Wei Ju

AU - Chen, Yen Ling

AU - Chu, Yi Wen

AU - Chien, Du Shieng

PY - 2019

Y1 - 2019

N2 - Background: Serum glutathione peroxidase-3 (GPx-3) is known as a key selenoprotein with antioxidant properties. GPx-3 deficiency has been associated with sepsis. The objectives of this study are (1) to compare the GPx-3 protein concentrations and GPx-3 bioactivity in normal healthy subjects and septic patients, and (2) to evaluate the relationship between GPx-3 bioactivity and its protein concentration. Methods: Serum samples were collected from 50 normal healthy subjects and 70 septic patients. The reliable bioanalytical methods for GPx-3 protein concentration and bioactivity in human serum were developed and validated. Analyses of GPx-3 bioactivity and GPx-3 protein concentration were then performed. Results: Geometric mean GPx-3 bioactivity was 78.13. U/l for patients with sepsis, significantly lower than normal subjects with 108.21. U/l (p <. 0.0001). Similarly, the GPx-3 protein concentration was significantly lower in patients with sepsis than in normal subjects, with the mean GPx-3 value of 0.78 vs 3.10. μg/ml, respectively (p <. 0.0001). A positive correlation was observed between the GPx-3 bioactivity and its corresponding protein concentration in septic serum samples (R = 0.74, p <. 0.0001), regardless of gender or age difference. Conclusion: These findings suggest that the decrease in GPx-3 bioactivity observed in the septic patients was resulted from the significant sepsis-related decline of GPx-3 protein concentrations.

AB - Background: Serum glutathione peroxidase-3 (GPx-3) is known as a key selenoprotein with antioxidant properties. GPx-3 deficiency has been associated with sepsis. The objectives of this study are (1) to compare the GPx-3 protein concentrations and GPx-3 bioactivity in normal healthy subjects and septic patients, and (2) to evaluate the relationship between GPx-3 bioactivity and its protein concentration. Methods: Serum samples were collected from 50 normal healthy subjects and 70 septic patients. The reliable bioanalytical methods for GPx-3 protein concentration and bioactivity in human serum were developed and validated. Analyses of GPx-3 bioactivity and GPx-3 protein concentration were then performed. Results: Geometric mean GPx-3 bioactivity was 78.13. U/l for patients with sepsis, significantly lower than normal subjects with 108.21. U/l (p <. 0.0001). Similarly, the GPx-3 protein concentration was significantly lower in patients with sepsis than in normal subjects, with the mean GPx-3 value of 0.78 vs 3.10. μg/ml, respectively (p <. 0.0001). A positive correlation was observed between the GPx-3 bioactivity and its corresponding protein concentration in septic serum samples (R = 0.74, p <. 0.0001), regardless of gender or age difference. Conclusion: These findings suggest that the decrease in GPx-3 bioactivity observed in the septic patients was resulted from the significant sepsis-related decline of GPx-3 protein concentrations.

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