The aim of this study is to evaluate the influence of serum [fetal bovine serum (FBS)] in cell culture medium on the nano and micro-sized ZnO toxicity to human lung epithelial cells (A549). Particles agglomeration and sedimentation state in medium were respectively measured by Zetasizer and UV-vis spectrometer. Methyl thiazolyl tetrazolium (MTT) and interleukin 8 (IL-8) analysis were selected for cytotoxicity assays. We found ZnO particles with a smaller primary size increased to a relative smaller secondary size in both serum/serum-free Dulbecco's modified Eagle's medium (DMEM), and caused the size-dependent cytotoxicity effect on ZnO nanoparticles. However, negative charges of serum caused the zeta potential of ZnO slightly decreased to -8 ~ -10 mV in DMEM, which resulted in smaller hydrodynamic size and much slower sedimentation than in serum free medium, leading to similarly lower toxicity.
|主出版物子標題||Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010|
|出版狀態||已發佈 - 2010|
|事件||Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 - Anaheim, CA, 美国|
持續時間: 六月 21 2010 → 六月 24 2010
|會議||Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010|
|期間||6/21/10 → 6/24/10|
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