Comparing cytotoxicity of ZnO nanoparticles in exposure to serum/serum-free cell culture medium

I. L. Hsiao, Y. J. Huang

研究成果: 書貢獻/報告類型會議貢獻

摘要

The aim of this study is to evaluate the influence of serum [fetal bovine serum (FBS)] in cell culture medium on the nano and micro-sized ZnO toxicity to human lung epithelial cells (A549). Particles agglomeration and sedimentation state in medium were respectively measured by Zetasizer and UV-vis spectrometer. Methyl thiazolyl tetrazolium (MTT) and interleukin 8 (IL-8) analysis were selected for cytotoxicity assays. We found ZnO particles with a smaller primary size increased to a relative smaller secondary size in both serum/serum-free Dulbecco's modified Eagle's medium (DMEM), and caused the size-dependent cytotoxicity effect on ZnO nanoparticles. However, negative charges of serum caused the zeta potential of ZnO slightly decreased to -8 ~ -10 mV in DMEM, which resulted in smaller hydrodynamic size and much slower sedimentation than in serum free medium, leading to similarly lower toxicity.
原文英語
主出版物標題Nanotechnology 2010
主出版物子標題Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
頁面557-560
頁數4
3
出版狀態已發佈 - 2010
對外發佈Yes
事件Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 - Anaheim, CA, 美国
持續時間: 六月 21 2010六月 24 2010

會議

會議Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010
國家美国
城市Anaheim, CA
期間6/21/106/24/10

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering
  • Electrical and Electronic Engineering

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    Hsiao, I. L., & Huang, Y. J. (2010). Comparing cytotoxicity of ZnO nanoparticles in exposure to serum/serum-free cell culture medium. 於 Nanotechnology 2010: Bio Sensors, Instruments, Medical, Environment and Energy - Technical Proceedings of the 2010 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2010 (卷 3, 頁 557-560)