摘要

Background: Silver nanoparticles (AgNPs) have been associated with the exacerbation of asthma; however, the immunological basis for the adjuvant effects of AgNPs is not well understood. Objective: The aim of the study reported here was to investigate the allergic effects of AgNP inhalation using proteomic approaches. Methods: Allergen provoked mice were exposed to 33 nm AgNPs at 3.3 mg/m3. Following this, bronchoalveolar lavage fluid (BALF) and plasma were collected to determine protein profiles. Results: In total, 106 and 79 AgNP-unique proteins were identified in the BALF of control and allergic mice, respectively. Additionally, 40 and 26 AgNP-unique proteins were found in the plasma of control and allergic mice, respectively. The BALF and plasma protein profiles suggested that metabolic, cellular, and immune system processes were associated with pulmonary exposure to AgNPs. In addition, we observed 18 proteins associated with systemic lupus erythematosus that were commonly expressed in both control and allergic mice after AgNP exposure. Significant allergy responses were observed after AgNP exposure in control and allergic mice, as determined by ovalbumin-specific immunoglobulin E. Conclusion: Inhaled AgNPs may regulate immune responses in the lungs of both control and allergic mice. Our results suggest that immunology is a vital response to AgNPs.
原文英語
頁(從 - 到)2783-2799
頁數17
期刊International Journal of Nanomedicine
8
DOIs
出版狀態已發佈 - 2013

指紋

Allergens
Silver
Proteomics
Nanoparticles
Proteins
Bronchoalveolar Lavage Fluid
Plasmas
Fluids
Immune System Phenomena
Immunology
Allergies
Immunologic Adjuvants
Immune system
Ovalbumin
Lung
Metabolome
Immunoglobulin E
Blood Proteins
Allergy and Immunology
Systemic Lupus Erythematosus

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Organic Chemistry
  • Drug Discovery

引用此文

@article{ba9645e4223c4ff486ce9f8bab316669,
title = "Comparative proteomics of inhaled silver nanoparticles in healthy and allergen provoked mice",
abstract = "Background: Silver nanoparticles (AgNPs) have been associated with the exacerbation of asthma; however, the immunological basis for the adjuvant effects of AgNPs is not well understood. Objective: The aim of the study reported here was to investigate the allergic effects of AgNP inhalation using proteomic approaches. Methods: Allergen provoked mice were exposed to 33 nm AgNPs at 3.3 mg/m3. Following this, bronchoalveolar lavage fluid (BALF) and plasma were collected to determine protein profiles. Results: In total, 106 and 79 AgNP-unique proteins were identified in the BALF of control and allergic mice, respectively. Additionally, 40 and 26 AgNP-unique proteins were found in the plasma of control and allergic mice, respectively. The BALF and plasma protein profiles suggested that metabolic, cellular, and immune system processes were associated with pulmonary exposure to AgNPs. In addition, we observed 18 proteins associated with systemic lupus erythematosus that were commonly expressed in both control and allergic mice after AgNP exposure. Significant allergy responses were observed after AgNP exposure in control and allergic mice, as determined by ovalbumin-specific immunoglobulin E. Conclusion: Inhaled AgNPs may regulate immune responses in the lungs of both control and allergic mice. Our results suggest that immunology is a vital response to AgNPs.",
keywords = "Bronchoalveolar lavage, Immunotoxicology, Proteome, Serum, Systemic lupus erythematosus",
author = "Su, {Chien Ling} and Chen, {Tzu Tao} and Chang, {Chih Cheng} and Chuang, {Kai Jen} and Wu, {Cheng Kuan} and Liu, {Wen Te} and Ho, {Kin Fai} and Lee, {Kang Yun} and Ho, {Shu Chuan} and Tseng, {Hsiu Er} and Chuang, {Hsiao Chi} and Cheng, {Tsun Jen}",
year = "2013",
doi = "10.2147/IJN.S46997",
language = "English",
volume = "8",
pages = "2783--2799",
journal = "International Journal of Nanomedicine",
issn = "1176-9114",
publisher = "Dove Medical Press Ltd.",

}

TY - JOUR

T1 - Comparative proteomics of inhaled silver nanoparticles in healthy and allergen provoked mice

AU - Su, Chien Ling

AU - Chen, Tzu Tao

AU - Chang, Chih Cheng

AU - Chuang, Kai Jen

AU - Wu, Cheng Kuan

AU - Liu, Wen Te

AU - Ho, Kin Fai

AU - Lee, Kang Yun

AU - Ho, Shu Chuan

AU - Tseng, Hsiu Er

AU - Chuang, Hsiao Chi

AU - Cheng, Tsun Jen

PY - 2013

Y1 - 2013

N2 - Background: Silver nanoparticles (AgNPs) have been associated with the exacerbation of asthma; however, the immunological basis for the adjuvant effects of AgNPs is not well understood. Objective: The aim of the study reported here was to investigate the allergic effects of AgNP inhalation using proteomic approaches. Methods: Allergen provoked mice were exposed to 33 nm AgNPs at 3.3 mg/m3. Following this, bronchoalveolar lavage fluid (BALF) and plasma were collected to determine protein profiles. Results: In total, 106 and 79 AgNP-unique proteins were identified in the BALF of control and allergic mice, respectively. Additionally, 40 and 26 AgNP-unique proteins were found in the plasma of control and allergic mice, respectively. The BALF and plasma protein profiles suggested that metabolic, cellular, and immune system processes were associated with pulmonary exposure to AgNPs. In addition, we observed 18 proteins associated with systemic lupus erythematosus that were commonly expressed in both control and allergic mice after AgNP exposure. Significant allergy responses were observed after AgNP exposure in control and allergic mice, as determined by ovalbumin-specific immunoglobulin E. Conclusion: Inhaled AgNPs may regulate immune responses in the lungs of both control and allergic mice. Our results suggest that immunology is a vital response to AgNPs.

AB - Background: Silver nanoparticles (AgNPs) have been associated with the exacerbation of asthma; however, the immunological basis for the adjuvant effects of AgNPs is not well understood. Objective: The aim of the study reported here was to investigate the allergic effects of AgNP inhalation using proteomic approaches. Methods: Allergen provoked mice were exposed to 33 nm AgNPs at 3.3 mg/m3. Following this, bronchoalveolar lavage fluid (BALF) and plasma were collected to determine protein profiles. Results: In total, 106 and 79 AgNP-unique proteins were identified in the BALF of control and allergic mice, respectively. Additionally, 40 and 26 AgNP-unique proteins were found in the plasma of control and allergic mice, respectively. The BALF and plasma protein profiles suggested that metabolic, cellular, and immune system processes were associated with pulmonary exposure to AgNPs. In addition, we observed 18 proteins associated with systemic lupus erythematosus that were commonly expressed in both control and allergic mice after AgNP exposure. Significant allergy responses were observed after AgNP exposure in control and allergic mice, as determined by ovalbumin-specific immunoglobulin E. Conclusion: Inhaled AgNPs may regulate immune responses in the lungs of both control and allergic mice. Our results suggest that immunology is a vital response to AgNPs.

KW - Bronchoalveolar lavage

KW - Immunotoxicology

KW - Proteome

KW - Serum

KW - Systemic lupus erythematosus

UR - http://www.scopus.com/inward/record.url?scp=84881270338&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84881270338&partnerID=8YFLogxK

U2 - 10.2147/IJN.S46997

DO - 10.2147/IJN.S46997

M3 - Article

C2 - 23946650

AN - SCOPUS:84881270338

VL - 8

SP - 2783

EP - 2799

JO - International Journal of Nanomedicine

JF - International Journal of Nanomedicine

SN - 1176-9114

ER -