Comparative proteomics, network analysis and post-translational modification identification reveal differential profiles of plasma Con A-bound glycoprotein biomarkers in gastric cancer

Yih Huei Uen, Kai Yuan Lin, Ding Ping Sun, Chen Chung Liao, Ming-Song Hsieh, Yung Kai Huang, Yen Wei Chen, Pei Hsuan Huang, Wei Jung Chen, Chih Chun Tai, Kuan Wei Lee, You Chia Chen, Ching Yu Lin

研究成果: 雜誌貢獻文章

41 引文 (Scopus)

摘要

In the study, we used Con A affinity chromatography, 1-D gel electrophoresis, and nano-LC-MS/MS to screen biomarker candidates in plasma samples obtained from 30 patients with gastric cancer and 30 healthy volunteers. First, we pooled plasma samples matched by age and sex. We identified 17 differentially expressed Con A-bound glycoproteins, including 10 upregulated proteins and 7 downregulated proteins; these differences were significant (Student's t-test, p-value41, trimethylation at aspartic acid-290, and flavin adenine dinucleotide at histidine-335. Biological significanceOur study was to describe a combinatorial approach of Con A affinity chromatography, 1-D SDS-PAGE, and nano-LC/MS/MS that provides a label-free, comparative glycoproteomic quantification strategy for the investigation of glycoprotein profiles in plasma from gastric cancer patients versus healthy volunteers and to identify glycoprotein biomarkers for the early clinical detection of gastric cancer. Three novel PTMs, HexHexNAc, trimethylation and FAD, in Con A-bound ITIH3 were identified and built in molecular modeling. The aspartic acid-290 trimethylation site was located in a metal ion-dependent adhesion site (MIDAS motif; 290-DXSXS. .T. .D-313) that may influence important function for binding protein ligands.
原文英語
頁(從 - 到)197-213
頁數17
期刊Journal of Proteomics
83
DOIs
出版狀態已發佈 - 五月 7 2013

指紋

Biomarkers
Post Translational Protein Processing
Electric network analysis
Proteomics
Stomach Neoplasms
Affinity chromatography
Glycoproteins
Flavin-Adenine Dinucleotide
Plasmas
Affinity Chromatography
Aspartic Acid
Healthy Volunteers
Pulse time modulation
Molecular modeling
Electrophoresis
Histidine
Metal ions
Labels
Polyacrylamide Gel Electrophoresis
Carrier Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

引用此文

Comparative proteomics, network analysis and post-translational modification identification reveal differential profiles of plasma Con A-bound glycoprotein biomarkers in gastric cancer. / Uen, Yih Huei; Lin, Kai Yuan; Sun, Ding Ping; Liao, Chen Chung; Hsieh, Ming-Song; Huang, Yung Kai; Chen, Yen Wei; Huang, Pei Hsuan; Chen, Wei Jung; Tai, Chih Chun; Lee, Kuan Wei; Chen, You Chia; Lin, Ching Yu.

於: Journal of Proteomics, 卷 83, 07.05.2013, p. 197-213.

研究成果: 雜誌貢獻文章

Uen, Yih Huei ; Lin, Kai Yuan ; Sun, Ding Ping ; Liao, Chen Chung ; Hsieh, Ming-Song ; Huang, Yung Kai ; Chen, Yen Wei ; Huang, Pei Hsuan ; Chen, Wei Jung ; Tai, Chih Chun ; Lee, Kuan Wei ; Chen, You Chia ; Lin, Ching Yu. / Comparative proteomics, network analysis and post-translational modification identification reveal differential profiles of plasma Con A-bound glycoprotein biomarkers in gastric cancer. 於: Journal of Proteomics. 2013 ; 卷 83. 頁 197-213.
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abstract = "In the study, we used Con A affinity chromatography, 1-D gel electrophoresis, and nano-LC-MS/MS to screen biomarker candidates in plasma samples obtained from 30 patients with gastric cancer and 30 healthy volunteers. First, we pooled plasma samples matched by age and sex. We identified 17 differentially expressed Con A-bound glycoproteins, including 10 upregulated proteins and 7 downregulated proteins; these differences were significant (Student's t-test, p-value41, trimethylation at aspartic acid-290, and flavin adenine dinucleotide at histidine-335. Biological significanceOur study was to describe a combinatorial approach of Con A affinity chromatography, 1-D SDS-PAGE, and nano-LC/MS/MS that provides a label-free, comparative glycoproteomic quantification strategy for the investigation of glycoprotein profiles in plasma from gastric cancer patients versus healthy volunteers and to identify glycoprotein biomarkers for the early clinical detection of gastric cancer. Three novel PTMs, HexHexNAc, trimethylation and FAD, in Con A-bound ITIH3 were identified and built in molecular modeling. The aspartic acid-290 trimethylation site was located in a metal ion-dependent adhesion site (MIDAS motif; 290-DXSXS. .T. .D-313) that may influence important function for binding protein ligands.",
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author = "Uen, {Yih Huei} and Lin, {Kai Yuan} and Sun, {Ding Ping} and Liao, {Chen Chung} and Ming-Song Hsieh and Huang, {Yung Kai} and Chen, {Yen Wei} and Huang, {Pei Hsuan} and Chen, {Wei Jung} and Tai, {Chih Chun} and Lee, {Kuan Wei} and Chen, {You Chia} and Lin, {Ching Yu}",
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T1 - Comparative proteomics, network analysis and post-translational modification identification reveal differential profiles of plasma Con A-bound glycoprotein biomarkers in gastric cancer

AU - Uen, Yih Huei

AU - Lin, Kai Yuan

AU - Sun, Ding Ping

AU - Liao, Chen Chung

AU - Hsieh, Ming-Song

AU - Huang, Yung Kai

AU - Chen, Yen Wei

AU - Huang, Pei Hsuan

AU - Chen, Wei Jung

AU - Tai, Chih Chun

AU - Lee, Kuan Wei

AU - Chen, You Chia

AU - Lin, Ching Yu

PY - 2013/5/7

Y1 - 2013/5/7

N2 - In the study, we used Con A affinity chromatography, 1-D gel electrophoresis, and nano-LC-MS/MS to screen biomarker candidates in plasma samples obtained from 30 patients with gastric cancer and 30 healthy volunteers. First, we pooled plasma samples matched by age and sex. We identified 17 differentially expressed Con A-bound glycoproteins, including 10 upregulated proteins and 7 downregulated proteins; these differences were significant (Student's t-test, p-value41, trimethylation at aspartic acid-290, and flavin adenine dinucleotide at histidine-335. Biological significanceOur study was to describe a combinatorial approach of Con A affinity chromatography, 1-D SDS-PAGE, and nano-LC/MS/MS that provides a label-free, comparative glycoproteomic quantification strategy for the investigation of glycoprotein profiles in plasma from gastric cancer patients versus healthy volunteers and to identify glycoprotein biomarkers for the early clinical detection of gastric cancer. Three novel PTMs, HexHexNAc, trimethylation and FAD, in Con A-bound ITIH3 were identified and built in molecular modeling. The aspartic acid-290 trimethylation site was located in a metal ion-dependent adhesion site (MIDAS motif; 290-DXSXS. .T. .D-313) that may influence important function for binding protein ligands.

AB - In the study, we used Con A affinity chromatography, 1-D gel electrophoresis, and nano-LC-MS/MS to screen biomarker candidates in plasma samples obtained from 30 patients with gastric cancer and 30 healthy volunteers. First, we pooled plasma samples matched by age and sex. We identified 17 differentially expressed Con A-bound glycoproteins, including 10 upregulated proteins and 7 downregulated proteins; these differences were significant (Student's t-test, p-value41, trimethylation at aspartic acid-290, and flavin adenine dinucleotide at histidine-335. Biological significanceOur study was to describe a combinatorial approach of Con A affinity chromatography, 1-D SDS-PAGE, and nano-LC/MS/MS that provides a label-free, comparative glycoproteomic quantification strategy for the investigation of glycoprotein profiles in plasma from gastric cancer patients versus healthy volunteers and to identify glycoprotein biomarkers for the early clinical detection of gastric cancer. Three novel PTMs, HexHexNAc, trimethylation and FAD, in Con A-bound ITIH3 were identified and built in molecular modeling. The aspartic acid-290 trimethylation site was located in a metal ion-dependent adhesion site (MIDAS motif; 290-DXSXS. .T. .D-313) that may influence important function for binding protein ligands.

KW - Concanavalin A

KW - Gastric cancer

KW - Glycoproteins

KW - Human plasma

KW - Mass spectrometry

KW - Post-translational modification

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