Comparative global immune-related gene profiling of somatic cells, human pluripotent stem cells and their derivatives: Implication for human lymphocyte proliferation

Chia Eng Wu, Chen Wei Yu, Kai Wei Chang, Wen Hsi Chou, Chen Yu Lu, Elisa Ghelfi, Fang Chun Wu, Pey Shynan Jan, Mei Chi Huang, Patrick Allard, Shau Ping Lin, Hong Nerng Ho, Hsin Fu Chen

研究成果: 雜誌貢獻文章

3 引文 斯高帕斯(Scopus)

摘要

Human pluripotent stem cells (hPSCs), including embryonic stem cells (ESCs) and induced PSCs (iPSCs), represent potentially unlimited cell sources for clinical applications. Previous studies have suggested that hPSCs may benefit from immune privilege and limited immunogenicity, as reflected by the reduced expression of major histocompatibility complex class-related molecules. Here we investigated the global immune-related gene expression profiles of human ESCs, hiPSCs and somatic cells and identified candidate immune-related genes that may alter their immunogenicity. The expression levels of global immune-related genes were determined by comparing undifferentiated and differentiated stem cells and three types of human somatic cells: dermal papilla cells, ovarian granulosa cells and foreskin fibroblast cells. We identified the differentially expressed genes CD24, GATA3, PROM1, THBS2, LY96, IFIT3, CXCR4, IL1R1, FGFR3, IDO1 and KDR, which overlapped with selected immune-related gene lists. In further analyses, mammalian target of rapamycin complex (mTOR) signaling was investigated in the differentiated stem cells following treatment with rapamycin and lentiviral transduction with specific short-hairpin RNAs. We found that the inhibition of mTOR signal pathways significantly downregulated the immunogenicity of differentiated stem cells. We also tested the immune responses induced in differentiated stem cells by mixed lymphocyte reactions. We found that CD24-and GATA3-deficient differentiated stem cells including neural lineage cells had limited abilities to activate human lymphocytes. By analyzing the transcriptome signature of immune-related genes, we observed a tendency of the hPSCs to differentiate toward an immune cell phenotype. Taken together, these data identify candidate immune-related genes that might constitute valuable targets for clinical applications. r The Author(s) 2017.
原文英語
文章編號e376
期刊Experimental and Molecular Medicine
49
發行號9
DOIs
出版狀態已發佈 - 九月 1 2017
對外發佈Yes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry

指紋 深入研究「Comparative global immune-related gene profiling of somatic cells, human pluripotent stem cells and their derivatives: Implication for human lymphocyte proliferation」主題。共同形成了獨特的指紋。

  • 引用此

    Wu, C. E., Yu, C. W., Chang, K. W., Chou, W. H., Lu, C. Y., Ghelfi, E., Wu, F. C., Jan, P. S., Huang, M. C., Allard, P., Lin, S. P., Ho, H. N., & Chen, H. F. (2017). Comparative global immune-related gene profiling of somatic cells, human pluripotent stem cells and their derivatives: Implication for human lymphocyte proliferation. Experimental and Molecular Medicine, 49(9), [e376]. https://doi.org/10.1038/emm.2017.134