Combined effects of polyethylene wear particle size and dosage on macrophage responses

Chih Hung Chang, Hsu Wei Fang, Yi Ching Ho, Chun Yen Lin, Chun Hsiung Huang, Charng Bin Yang

研究成果: 雜誌貢獻文章同行評審

摘要

Ultrahigh molecular weight polyethylene (UHMWPE) wear particles-induced osteolysis and aseptic loosening is the main reason causing the failure of the total joint replacement. It is believed that cytokines, such as tumor necrosis factor-α (TNF-α), released by macrophages that are stimulated by UHMWPE particles can activate osteoclasts and facilitate osteolysis. In one of our previous studies, we have attempted to explain the effects of wear particles on bioactivity by analyzing the phagocytosis of particles from a biophysical viewpoint. By integrating the membrane surface areas of macrophages, we were able to estimate the maximum number of internalized particles, which is defined as the phagocytic capacity for a particle population. It has been found that for the particles with same total volume, the elongated particles have a smaller phagocytic capacity in comparison to the spherical particles. By further analyzing the cell culture and results of animal tests as reported in the literature, we found that the ratio of the particle concentration (no. of particles/no. of cells) to the phagocytic capacity is a critical value for the particles-induced biological responses. When this ratio is smaller than unity, the bioactivity is approximately proportional to the ratio itself; but limited change of biological response was observed when this ratio is larger than unity. In this study, we obtained further experimental evidence in support of this hypothesis. The proliferation and TNF-α release of macrophages when co-cultured with different dosages of UHMWPE particles in an inverted in vitro cell culture model were measured.
原文英語
頁(從 - 到)279-291
頁數13
期刊Biomedical Engineering - Applications, Basis and Communications
22
發行號4
DOIs
出版狀態已發佈 - 八月 1 2010
對外發佈

ASJC Scopus subject areas

  • 生物工程
  • 生物物理學
  • 生物醫學工程

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