Combination treatment with luteolin and quercetin enhances antiproliferative effects in nicotine-treated MDA-MB-231 cells by down-regulating nicotinic acetylcholine receptors

Yung Leun Shih, Hui Ching Liu, Ching Shyang Chen, Chung-Huei Hsu, Min Hsiung Pan, Hui Wen Chang, Chien Hsi Chang, Feng Chia Chen, Chi Tang Ho, Yi-Yuan Yang, Yuan-Soon Ho

研究成果: 雜誌貢獻文章同行評審

54 引文 斯高帕斯(Scopus)

摘要

Large-scale epidemiological cohort studies performed in the United States indicate that breast cancer risk is associated with active and passive smoking. As of yet, however, there is no direct evidence of antitumor effects by agents that block the effect of tobacco compound nicotine (Nie) on relevant nicotinic receptors (nAChR) involved in breast tumorigenesis. In the present study, the expression profiles of different nAChR subunits in the human breast cancer cell line (MDA-MB-231) were characterized by RT-PCR. Nic (>0.1 μ, 6 h) significantly increased α9-nAChR mRNA and protein expression levels in human breast cancer cells (MDA-MB-231 cells). On the other hand, combined treatment with luteolin (Lut, 0.5 μ and quercetin (Que, 0.5 μ.M) profoundly decreased MDA-MB-231 proliferation by down-regulating α9-nAChR expression. MDA-MB-231 cells were cultured in soft agar to evaluate anchorage-independent colony formation; combined treatment of Lut + Que inhibited Nic-induced MDA-MB-231 colony formation. Interestingly, the number of colonies formed was profoundly reduced in α9-nAChR knockdown (Si α9) cells in the combined (Lut + Que)-treated group as compared to the relevant control groups. Such results show that Lut- or Que-induced antitransforming activities were not limited to specific inhibition of the α9-nAChR receptor. Both α5- and α9-nAChR appear to be important molecular targets for Lut- and Que-induced antitumor effects in human breast cancer cells.
原文英語
頁(從 - 到)235-241
頁數7
期刊Journal of Agricultural and Food Chemistry
58
發行號1
DOIs
出版狀態已發佈 - 一月 13 2010

ASJC Scopus subject areas

  • 農業與生物科學 (全部)
  • 化學 (全部)

指紋

深入研究「Combination treatment with luteolin and quercetin enhances antiproliferative effects in nicotine-treated MDA-MB-231 cells by down-regulating nicotinic acetylcholine receptors」主題。共同形成了獨特的指紋。

引用此