Colloidal crystal based plasma polymer patterning to control Pseudomonas aeruginosa attachment to surfaces

Hitesh Pingle, Peng Yuan Wang, Helmut Thissen, Sally McArthur, Peter Kingshott

研究成果: 雜誌貢獻文章同行評審

10 引文 斯高帕斯(Scopus)

摘要

Biofilm formation on medical implants and subsequent infections are a global problem. A great deal of effort has focused on developing chemical contrasts based on micro- and nanopatterning for studying and controlling cells and bacteria at surfaces. It has been known that micro- and nanopatterns on surfaces can influence biomolecule adsorption, and subsequent cell and bacterial adhesion. However, less focus has been on precisely controlling patterns to study the initial bacterial attachment mechanisms and subsequently how the patterning influences the role played by biomolecular adsorption on biofilm formation. In this work, the authors have used colloidal self-assembly in a confined area to pattern surfaces with colloidal crystals and used them as masks during allylamine plasma polymer (AAMpp) deposition to generate highly ordered patterns from the micro- to the nanoscale. Polyethylene glycol (PEG)-aldehyde was grafted to the plasma regions via "cloud point" grafting to prevent the attachment of bacteria on the plasma patterned surface regions, thereby controlling the adhesive sites by choice of the colloidal crystal morphology. Pseudomonas aeruginosa was chosen to study the bacterial interactions with these chemically patterned surfaces. Scanning electron microscope, x-ray photoelectron spectroscopy (XPS), atomic force microscopy, and epifluorescence microscopy were used for pattern characterization, surface chemical analysis, and imaging of attached bacteria. The AAMpp influenced bacterial attachment because of the amine groups displaying a positive charge. XPS results confirm the successful grafting of PEG on the AAMpp surfaces. The results showed that PEG patterns can be used as a surface for bacterial patterning including investigating the role of biomolecular patterning on bacterial attachment. These types of patterns are easy to fabricate and could be useful in further applications in biomedical research.
原文英語
期刊Biointerphases
10
發行號4
DOIs
出版狀態已發佈 - 2015
對外發佈

ASJC Scopus subject areas

  • 化學 (全部)
  • 材料科學(全部)
  • 生物化學、遺傳與分子生物學 (全部)
  • 物理與天文學 (全部)

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