Coculture of endothelial and smooth muscle cells on a collagen membrane in the development of a small-diameter vascular graft

Hsi Chin Wu, Tzu Wei Wang, Pei Leun Kang, Yang Hwei Tsuang, Jui Sheng Sun, Feng Huei Lin

研究成果: 雜誌貢獻文章同行評審

79 引文 斯高帕斯(Scopus)

摘要

In this study, we have evaluated the feasibility of developing a biodegradable collagenous small diameter vascular graft of 2 mm diameter and 1 cm length. In brief, bi-layer type I collagen membrane was fabricated under vacuum suction and lyophilization methods. The smooth muscle cells were inoculated into the lower side of the porous membrane, while endothelial cells were seeded onto upper smooth side of the membrane. After cultured for 7 days, the vascular substitute was either harvested for in vitro examination or in vivo implanted in the subcutaneous layer for biocompatibility test. The tubular vascular prosthesis was then used as a temporary absorbable guide that served as an in vivo vascular graft to promote the complete regeneration of rat inferior vena cava. After implantation for 12 weeks, a thin continuous layer of endothelial cells and smooth muscle cells were lined with the vascular lumen and tunic media, respectively. Histology results showed that there were no signs of significant thrombogeneity and intima hyperplasia. This tissue engineered vascular substitute not only had enough tensile strength and good biocompatibility, but also advanced vascular regeneration. In the future, we suggest that this biodegradable vascular substitute will provide with the possibility in application on small diameter prosthetic grafts in artificial blood vessels.

原文英語
頁(從 - 到)1385-1392
頁數8
期刊Biomaterials
28
發行號7
DOIs
出版狀態已發佈 - 三月 2007
對外發佈Yes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

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