Cocoa procyanidins inhibit proliferation and angiogenic signals in human dermal microvascular endothelial cells following stimulation by low-level H 2O2

Thomas P. Kenny, Carl L. Keen, Paul Jones, Hsing Jien Kung, Harold H. Schmitz, M. Eric Gershwin

研究成果: 雜誌貢獻文章

17 引文 (Scopus)

摘要

Procyanidins extracted from cocoa play a role in the defense against oxidative stress, as well as in vascular and immune functions. We previously reported that pentameric procyanidins isolated from cocoa inhibit the expression of the tyrosine kinase ErbB2 gene, thus slowing the growth of cultured human aortic endothelial cells. We herein investigate the further consequences of such inhibition by cocoa procyanidins, particularly regarding the protein level in phosphorylation patterns and the effects on the proliferation of human dermal microvascular endothelial cells (HDMECs) following angiogenic stimulation with low-level H2O2. We report herein that both the pentameric and octameric procyanidin fractions of cocoa inhibit the proliferation of HDMECs, whereas the pentameric fraction modulates the activity of several crucial proteins in angiogenic signaling by altering their tyrosine phosphorylation. Similar to aortic endothelial cells, the pentameric procyanidin fraction down-regulates the expression of ErbB2 tyrosine kinase in HDMECs. In conclusion, we report evidence suggesting that polyphenols may influence endothelial growth signaling, thus affecting angiogenesis in vitro. If these observations are applicable in vivo, they suggest a beneficial effect for cells overexpressing ErbB2, such as in speclfic neopiaslas.
原文英語
頁(從 - 到)765-771
頁數7
期刊Experimental Biology and Medicine
229
發行號8
DOIs
出版狀態已發佈 - 一月 1 2004
對外發佈Yes

指紋

Cocoa
Proanthocyanidins
Endothelial cells
Endothelial Cells
Skin
Phosphorylation
Protein-Tyrosine Kinases
Angiogenic Proteins
erbB-2 Genes
Oxidative stress
Polyphenols
Growth
Blood Vessels
Tyrosine
Proteins
Oxidative Stress
Down-Regulation
Genes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

引用此文

Cocoa procyanidins inhibit proliferation and angiogenic signals in human dermal microvascular endothelial cells following stimulation by low-level H 2O2. / Kenny, Thomas P.; Keen, Carl L.; Jones, Paul; Kung, Hsing Jien; Schmitz, Harold H.; Gershwin, M. Eric.

於: Experimental Biology and Medicine, 卷 229, 編號 8, 01.01.2004, p. 765-771.

研究成果: 雜誌貢獻文章

Kenny, Thomas P. ; Keen, Carl L. ; Jones, Paul ; Kung, Hsing Jien ; Schmitz, Harold H. ; Gershwin, M. Eric. / Cocoa procyanidins inhibit proliferation and angiogenic signals in human dermal microvascular endothelial cells following stimulation by low-level H 2O2. 於: Experimental Biology and Medicine. 2004 ; 卷 229, 編號 8. 頁 765-771.
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