Coalescence of RAGE in lipid rafts in response to cytolethal distending toxin-induced inflammation

Hwai Jeng Lin, Zhi Pei Jiang, Horng Ren Lo, Chun Lung Feng, Yu Chen, Chih Jung Chen, Chia Yu Yang, Mei Zi Huang, Hui Yu Wu, Yu An Chen, Cheng Hsun Chiu, Chih Ho Lai

研究成果: 雜誌貢獻文章同行評審

6 引文 斯高帕斯(Scopus)

摘要

The receptor for advanced glycation end products (RAGE) interacts with various molecules in the cell membrane to induce an inflammatory response. The cytolethal distending toxin (CDT) produced by Campylobacter jejuni contains three subunits: CdtA, CdtB, and CdtC. Amongst, CdtA and CdtC interact with membrane lipid rafts, by which CdtB enters the nucleus to induce pathogenesis. In this study, we first explored the relationships between RAGE, lipid rafts, and inflammation in gastrointestinal epithelial cells exposed to CDT. Our results showed that CDT activated the expression of RAGE and high mobility group box 1 (HMGB1), followed by the recruitment of RAGE into lipid rafts. In contrast, RAGE antagonist inhibited CDT-induced inflammation via the RAGE-HMGB1 axis. Disruption of lipid rafts decreased CDT-induced downstream signaling, which in turn attenuated the inflammatory response. Furthermore, in vivo studies revealed severe inflammation and upregulation of RAGE and IL-1β in the intestinal tissues of CDT-treated mice. These results demonstrate that mobilization of RAGE to lipid rafts plays a crucial role in CDT-induced inflammation.

原文英語
文章編號109
期刊Frontiers in Immunology
10
發行號FEB
DOIs
出版狀態已發佈 - 一月 1 2019

ASJC Scopus subject areas

  • 免疫學和過敏
  • 免疫學

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