Cloning, purification, crystallization and preliminary X-ray analysis of XC229, a conserved hypothetical protein from Xanthomonas campestris

Ko Hsin Chin, Wei Tien Kuo, Chia Cheng Chou, Hui Lin Shr, Ping Chiang Lyu, Andrew H.J. Wang, Shan Ho Chou

研究成果: 雜誌貢獻文章同行評審

1 引文 斯高帕斯(Scopus)

摘要

Xanthomonas campestris pv. campestris is a Gram-negative yellow-pigmented pathogenic bacterium that causes black rot, one of the major worldwide diseases of cruciferous crops. Its genome contains approximately 4500 genes, roughly one third of which have no known structure and/or function. However, some of these unknown genes are highly conserved among several different bacterial genuses. XC229 is one such protein containing 134 amino acids. It was overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. The crystal diffracted to a resolution of at least 1.80 Å. It is cubic and belongs to space group I2x3, with unit-cell parameters a = b = c = 106.8 Å. It contains one or two molecules per asymmetric unit.

原文英語
頁(從 - 到)694-696
頁數3
期刊Acta Crystallographica Section F: Structural Biology and Crystallization Communications
61
發行號7
DOIs
出版狀態已發佈 - 7月 2005
對外發佈

ASJC Scopus subject areas

  • 生物物理學
  • 結構生物學
  • 生物化學
  • 遺傳學
  • 凝聚態物理學

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