摘要
The mRNAs were extracted from common carp (Cyprinus carpio) lenses, purified, reverse transcribed, dC tailed and cloned into Escherichia coli with pBR322 as vector. The cloning efficiency was around 1·107 colonies per μg of mRNA. A clone (pC20) was found by hybrid-arrested translation to contain the cDNA related to carp crystallins. However, comparison of the derived amino-acid sequence with bovine γ-II and βs-crystallins indicates that this carp crystallin sequence resembles closely the bovine βs-crystallin and should be better classified as such except that this fish sequence does not contain the N-terminal 'arm' of four amino-acid residues present in bovine βs-crystallin.
原文 | 英語 |
---|---|
頁(從 - 到) | 89-92 |
頁數 | 4 |
期刊 | BBA - Gene Structure and Expression |
卷 | 910 |
發行號 | 1 |
DOIs | |
出版狀態 | 已發佈 - 10月 9 1987 |
對外發佈 | 是 |
ASJC Scopus subject areas
- 結構生物學
- 生物物理學
- 生物化學
- 遺傳學