Cloning and sequencing of a carp βs-crystallin cDNA

Tschining Chang; Wen Chang Chang

doi:10.1016/0167-4781(87)90098-4

Cloning and sequencing of a carp β_s-crystallin cDNA

Tschining Chang, Wen Chang Chang

研究成果: 雜誌貢獻 › 文章 › 同行評審

27 引文斯高帕斯（Scopus）

摘要

The mRNAs were extracted from common carp (Cyprinus carpio) lenses, purified, reverse transcribed, dC tailed and cloned into Escherichia coli with pBR322 as vector. The cloning efficiency was around 1·107 colonies per μg of mRNA. A clone (pC20) was found by hybrid-arrested translation to contain the cDNA related to carp crystallins. However, comparison of the derived amino-acid sequence with bovine γ-II and βs-crystallins indicates that this carp crystallin sequence resembles closely the bovine βs-crystallin and should be better classified as such except that this fish sequence does not contain the N-terminal 'arm' of four amino-acid residues present in bovine βs-crystallin.

原文	英語
頁（從 - 到）	89-92
頁數	4
期刊	BBA - Gene Structure and Expression
卷	910
發行號	1
DOIs	https://doi.org/10.1016/0167-4781(87)90098-4
出版狀態	已發佈 - 十月 9 1987
對外發佈	Yes

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

存取文件

10.1016/0167-4781(87)90098-4

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引用此

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abstract = "The mRNAs were extracted from common carp (Cyprinus carpio) lenses, purified, reverse transcribed, dC tailed and cloned into Escherichia coli with pBR322 as vector. The cloning efficiency was around 1·107 colonies per μg of mRNA. A clone (pC20) was found by hybrid-arrested translation to contain the cDNA related to carp crystallins. However, comparison of the derived amino-acid sequence with bovine γ-II and βs-crystallins indicates that this carp crystallin sequence resembles closely the bovine βs-crystallin and should be better classified as such except that this fish sequence does not contain the N-terminal 'arm' of four amino-acid residues present in bovine βs-crystallin.",

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AU - Chang, Wen Chang

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AB - The mRNAs were extracted from common carp (Cyprinus carpio) lenses, purified, reverse transcribed, dC tailed and cloned into Escherichia coli with pBR322 as vector. The cloning efficiency was around 1·107 colonies per μg of mRNA. A clone (pC20) was found by hybrid-arrested translation to contain the cDNA related to carp crystallins. However, comparison of the derived amino-acid sequence with bovine γ-II and βs-crystallins indicates that this carp crystallin sequence resembles closely the bovine βs-crystallin and should be better classified as such except that this fish sequence does not contain the N-terminal 'arm' of four amino-acid residues present in bovine βs-crystallin.

KW - (Carp lens)

KW - cDNA cloning

KW - Nucleotide sequence

KW - β-Crystallin

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