Cloning and sequencing of a carp βs-crystallin cDNA

Tschining Chang, Wen Chang Chang

研究成果: 雜誌貢獻文章同行評審

27 引文 斯高帕斯(Scopus)

摘要

The mRNAs were extracted from common carp (Cyprinus carpio) lenses, purified, reverse transcribed, dC tailed and cloned into Escherichia coli with pBR322 as vector. The cloning efficiency was around 1·107 colonies per μg of mRNA. A clone (pC20) was found by hybrid-arrested translation to contain the cDNA related to carp crystallins. However, comparison of the derived amino-acid sequence with bovine γ-II and βs-crystallins indicates that this carp crystallin sequence resembles closely the bovine βs-crystallin and should be better classified as such except that this fish sequence does not contain the N-terminal 'arm' of four amino-acid residues present in bovine βs-crystallin.
原文英語
頁(從 - 到)89-92
頁數4
期刊BBA - Gene Structure and Expression
910
發行號1
DOIs
出版狀態已發佈 - 十月 9 1987
對外發佈Yes

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

指紋 深入研究「Cloning and sequencing of a carp β<sub>s</sub>-crystallin cDNA」主題。共同形成了獨特的指紋。

引用此