Cloning and characterization of the carp prolactin gene

Huang Tsu Chen, Chien Shun Chiou, Wen Chang Chang

研究成果: 雜誌貢獻文章

17 引文 (Scopus)

摘要

A carp genomic DNA clone containing the carp prolactin (Prl) gene was isolated with carp Prl cDNA as a probe. The organization of the carp Prl gene was determined by restriction nuclease mapping and nucleotide sequencing. The Prl gene comprises approx. 2.8 kilobasepairs (kb) of DNA including the 5′-flanking region, five exons, four introns and the 3′-flanking region. Analysis of the 5′-flanking region reveals (1) the sequence TATATAAT at positions -38 to -31 upstream from the cap site which was found to be a guanine residue, and (2) the palindrome, CTCATTGCATA-TACAAATGAG at positions -79 to -59. The carp Prl gene matches with the reported cDNA except for one difference in coding region and five in the 3′-flanking region, while the encoded amino acid sequences are identical. The arrangement of exons and introns is very similar to that seen in carp GH as well as mammalian Prl, which, however, have much longer introns.
原文英語
頁(從 - 到)315-318
頁數4
期刊BBA - Gene Structure and Expression
1088
發行號2
DOIs
出版狀態已發佈 - 二月 16 1991
對外發佈Yes

指紋

Carps
Cloning
Prolactin
Organism Cloning
Genes
Introns
3' Flanking Region
5' Flanking Region
Exons
Complementary DNA
Restriction Mapping
DNA
Guanine
Amino Acid Sequence
Nucleotides
Clone Cells
Amino Acids

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

引用此文

Cloning and characterization of the carp prolactin gene. / Chen, Huang Tsu; Chiou, Chien Shun; Chang, Wen Chang.

於: BBA - Gene Structure and Expression, 卷 1088, 編號 2, 16.02.1991, p. 315-318.

研究成果: 雜誌貢獻文章

Chen, Huang Tsu ; Chiou, Chien Shun ; Chang, Wen Chang. / Cloning and characterization of the carp prolactin gene. 於: BBA - Gene Structure and Expression. 1991 ; 卷 1088, 編號 2. 頁 315-318.
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N2 - A carp genomic DNA clone containing the carp prolactin (Prl) gene was isolated with carp Prl cDNA as a probe. The organization of the carp Prl gene was determined by restriction nuclease mapping and nucleotide sequencing. The Prl gene comprises approx. 2.8 kilobasepairs (kb) of DNA including the 5′-flanking region, five exons, four introns and the 3′-flanking region. Analysis of the 5′-flanking region reveals (1) the sequence TATATAAT at positions -38 to -31 upstream from the cap site which was found to be a guanine residue, and (2) the palindrome, CTCATTGCATA-TACAAATGAG at positions -79 to -59. The carp Prl gene matches with the reported cDNA except for one difference in coding region and five in the 3′-flanking region, while the encoded amino acid sequences are identical. The arrangement of exons and introns is very similar to that seen in carp GH as well as mammalian Prl, which, however, have much longer introns.

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