Clinicopathological Correlation and Prognostic Significance of Protein Kinase Cα Overexpression in Human Gastric Carcinoma

Shee Chan Lin, Wei Yu Chen, Kai Yuan Lin, Sheng-Hsuan Chen, Chun Chao Chang, Sey-En Lin, Chia Lang Fang

研究成果: 雜誌貢獻文章

8 引文 (Scopus)

摘要

Objectives: This study investigated the PKCα protein expression in gastric carcinoma, and correlated it with clinicopathological parameters. The prognostic significance of PKCα protein expression in gastric carcinoma was analyzed. Methods: Quantitative real-time PCR test was applied to compare the PKCα mRNA expression in tumorous and nontumorous tissues of gastric carcinoma in ten randomly selected cases. Then PKCα protein expression was evaluated in 215 cases of gastric carcinoma using immunohistochemical method. The immunoreactivity was scored semiquantitatively as: 0 = absent; 1 = weak; 2 = moderate; and 3 = strong. All cases were further classified into two groups, namely PKCα overexpression group with score 2 or 3, and non-overexpression group with score 0 or 1. The PKCα protein expression was correlated with clinicopathological parameters. Survival analysis was performed to determine the prognostic significance of PKCα protein expression in patients with gastric carcinoma. Results: PKCα mRNA expression was upregulated in all ten cases of gastric carcinoma via quantitative real-time PCR test. In immunohistochemical study, eighty-eight out of 215 cases (41%) of gastric carcinoma revealed PKCα protein overexpression, which was statistically correlated with age (P = 0.0073), histologic type (P
原文英語
文章編號e56675
期刊PLoS One
8
發行號2
DOIs
出版狀態已發佈 - 二月 26 2013

指紋

protein kinase C
Protein Kinase C
carcinoma
Stomach
stomach
Carcinoma
protein synthesis
Proteins
Real-Time Polymerase Chain Reaction
quantitative polymerase chain reaction
Messenger RNA
Survival Analysis
quantitative analysis
Tissue
testing
proteins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

引用此文

Clinicopathological Correlation and Prognostic Significance of Protein Kinase Cα Overexpression in Human Gastric Carcinoma. / Lin, Shee Chan; Chen, Wei Yu; Lin, Kai Yuan; Chen, Sheng-Hsuan; Chang, Chun Chao; Lin, Sey-En; Fang, Chia Lang.

於: PLoS One, 卷 8, 編號 2, e56675, 26.02.2013.

研究成果: 雜誌貢獻文章

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AU - Lin, Shee Chan

AU - Chen, Wei Yu

AU - Lin, Kai Yuan

AU - Chen, Sheng-Hsuan

AU - Chang, Chun Chao

AU - Lin, Sey-En

AU - Fang, Chia Lang

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N2 - Objectives: This study investigated the PKCα protein expression in gastric carcinoma, and correlated it with clinicopathological parameters. The prognostic significance of PKCα protein expression in gastric carcinoma was analyzed. Methods: Quantitative real-time PCR test was applied to compare the PKCα mRNA expression in tumorous and nontumorous tissues of gastric carcinoma in ten randomly selected cases. Then PKCα protein expression was evaluated in 215 cases of gastric carcinoma using immunohistochemical method. The immunoreactivity was scored semiquantitatively as: 0 = absent; 1 = weak; 2 = moderate; and 3 = strong. All cases were further classified into two groups, namely PKCα overexpression group with score 2 or 3, and non-overexpression group with score 0 or 1. The PKCα protein expression was correlated with clinicopathological parameters. Survival analysis was performed to determine the prognostic significance of PKCα protein expression in patients with gastric carcinoma. Results: PKCα mRNA expression was upregulated in all ten cases of gastric carcinoma via quantitative real-time PCR test. In immunohistochemical study, eighty-eight out of 215 cases (41%) of gastric carcinoma revealed PKCα protein overexpression, which was statistically correlated with age (P = 0.0073), histologic type (P

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