Clinical significance of signal pattern of high-risk human papillomavirus using a novel fluorescence in situ hybridization assay in cervical cytology

C. M. Ho, B. H. Lee, S. F. Chang, T. Y. Chien, S. H. Huang, C. C. Yan, W. F. Cheng

研究成果: 雜誌貢獻文章

3 引文 (Scopus)

摘要

The present study aimed to evaluate a novel fluorescence in situ hybridization (FISH) assay for detecting the high-risk human papillomavirus (HR-HPV) DNA and signal pattern in cervical cytology specimens and for identifying cervical intraepithelial neoplasia (CIN) lesions. One hundred and ninety-six liquid-based cytology specimens with CIN were recruited. The signal pattern (punctate, mixed punctate and diffuse, and diffuse) detected by FISH was compared with E6 mRNA and correlated with histological classification. FISH and E6-type specific polymerase chain reaction (PCR) had fair to good agreement for detecting HPV DNA across all grades of CIN (kappa coefficient, 0.37-0.73). Among 44 samples of negative FISH and positive E6 type-specific PCR in HPV 16, 18, 31, 33, 52 and 58, 82% (36/44) of E6 mRNA were not detected, in contrast to 41% (48/118) of positive FISH and positive E6 type-specific PCR (p

原文英語
頁(從 - 到)386-394
頁數9
期刊Clinical Microbiology and Infection
17
發行號3
DOIs
出版狀態已發佈 - 三月 2011

指紋

Fluorescence In Situ Hybridization
Cell Biology
Cervical Intraepithelial Neoplasia
Polymerase Chain Reaction
Human papillomavirus 18
Messenger RNA
Human papillomavirus 16
DNA

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

引用此文

Clinical significance of signal pattern of high-risk human papillomavirus using a novel fluorescence in situ hybridization assay in cervical cytology. / Ho, C. M.; Lee, B. H.; Chang, S. F.; Chien, T. Y.; Huang, S. H.; Yan, C. C.; Cheng, W. F.

於: Clinical Microbiology and Infection, 卷 17, 編號 3, 03.2011, p. 386-394.

研究成果: 雜誌貢獻文章

@article{b9ab2c9da9c14007be2f1626029a3587,
title = "Clinical significance of signal pattern of high-risk human papillomavirus using a novel fluorescence in situ hybridization assay in cervical cytology",
abstract = "The present study aimed to evaluate a novel fluorescence in situ hybridization (FISH) assay for detecting the high-risk human papillomavirus (HR-HPV) DNA and signal pattern in cervical cytology specimens and for identifying cervical intraepithelial neoplasia (CIN) lesions. One hundred and ninety-six liquid-based cytology specimens with CIN were recruited. The signal pattern (punctate, mixed punctate and diffuse, and diffuse) detected by FISH was compared with E6 mRNA and correlated with histological classification. FISH and E6-type specific polymerase chain reaction (PCR) had fair to good agreement for detecting HPV DNA across all grades of CIN (kappa coefficient, 0.37-0.73). Among 44 samples of negative FISH and positive E6 type-specific PCR in HPV 16, 18, 31, 33, 52 and 58, 82{\%} (36/44) of E6 mRNA were not detected, in contrast to 41{\%} (48/118) of positive FISH and positive E6 type-specific PCR (p",
keywords = "Cervical intraepithelial neoplasm, Fluorescence in situ hybridization, High-risk human papillomavirus, Signal pattern",
author = "Ho, {C. M.} and Lee, {B. H.} and Chang, {S. F.} and Chien, {T. Y.} and Huang, {S. H.} and Yan, {C. C.} and Cheng, {W. F.}",
year = "2011",
month = "3",
doi = "10.1111/j.1469-0691.2010.03186.x",
language = "English",
volume = "17",
pages = "386--394",
journal = "Clinical Microbiology and Infection",
issn = "1198-743X",
publisher = "Elsevier Limited",
number = "3",

}

TY - JOUR

T1 - Clinical significance of signal pattern of high-risk human papillomavirus using a novel fluorescence in situ hybridization assay in cervical cytology

AU - Ho, C. M.

AU - Lee, B. H.

AU - Chang, S. F.

AU - Chien, T. Y.

AU - Huang, S. H.

AU - Yan, C. C.

AU - Cheng, W. F.

PY - 2011/3

Y1 - 2011/3

N2 - The present study aimed to evaluate a novel fluorescence in situ hybridization (FISH) assay for detecting the high-risk human papillomavirus (HR-HPV) DNA and signal pattern in cervical cytology specimens and for identifying cervical intraepithelial neoplasia (CIN) lesions. One hundred and ninety-six liquid-based cytology specimens with CIN were recruited. The signal pattern (punctate, mixed punctate and diffuse, and diffuse) detected by FISH was compared with E6 mRNA and correlated with histological classification. FISH and E6-type specific polymerase chain reaction (PCR) had fair to good agreement for detecting HPV DNA across all grades of CIN (kappa coefficient, 0.37-0.73). Among 44 samples of negative FISH and positive E6 type-specific PCR in HPV 16, 18, 31, 33, 52 and 58, 82% (36/44) of E6 mRNA were not detected, in contrast to 41% (48/118) of positive FISH and positive E6 type-specific PCR (p

AB - The present study aimed to evaluate a novel fluorescence in situ hybridization (FISH) assay for detecting the high-risk human papillomavirus (HR-HPV) DNA and signal pattern in cervical cytology specimens and for identifying cervical intraepithelial neoplasia (CIN) lesions. One hundred and ninety-six liquid-based cytology specimens with CIN were recruited. The signal pattern (punctate, mixed punctate and diffuse, and diffuse) detected by FISH was compared with E6 mRNA and correlated with histological classification. FISH and E6-type specific polymerase chain reaction (PCR) had fair to good agreement for detecting HPV DNA across all grades of CIN (kappa coefficient, 0.37-0.73). Among 44 samples of negative FISH and positive E6 type-specific PCR in HPV 16, 18, 31, 33, 52 and 58, 82% (36/44) of E6 mRNA were not detected, in contrast to 41% (48/118) of positive FISH and positive E6 type-specific PCR (p

KW - Cervical intraepithelial neoplasm

KW - Fluorescence in situ hybridization

KW - High-risk human papillomavirus

KW - Signal pattern

UR - http://www.scopus.com/inward/record.url?scp=79751480818&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79751480818&partnerID=8YFLogxK

U2 - 10.1111/j.1469-0691.2010.03186.x

DO - 10.1111/j.1469-0691.2010.03186.x

M3 - Article

C2 - 20148921

AN - SCOPUS:79751480818

VL - 17

SP - 386

EP - 394

JO - Clinical Microbiology and Infection

JF - Clinical Microbiology and Infection

SN - 1198-743X

IS - 3

ER -