Clinical significance of signal pattern of high-risk human papillomavirus using a novel fluorescence in situ hybridization assay in cervical cytology

C. M. Ho, B. H. Lee, S. F. Chang, T. Y. Chien, S. H. Huang, C. C. Yan, W. F. Cheng

研究成果: 雜誌貢獻文章

3 引文 斯高帕斯(Scopus)

摘要

The present study aimed to evaluate a novel fluorescence in situ hybridization (FISH) assay for detecting the high-risk human papillomavirus (HR-HPV) DNA and signal pattern in cervical cytology specimens and for identifying cervical intraepithelial neoplasia (CIN) lesions. One hundred and ninety-six liquid-based cytology specimens with CIN were recruited. The signal pattern (punctate, mixed punctate and diffuse, and diffuse) detected by FISH was compared with E6 mRNA and correlated with histological classification. FISH and E6-type specific polymerase chain reaction (PCR) had fair to good agreement for detecting HPV DNA across all grades of CIN (kappa coefficient, 0.37-0.73). Among 44 samples of negative FISH and positive E6 type-specific PCR in HPV 16, 18, 31, 33, 52 and 58, 82% (36/44) of E6 mRNA were not detected, in contrast to 41% (48/118) of positive FISH and positive E6 type-specific PCR (p

原文英語
頁(從 - 到)386-394
頁數9
期刊Clinical Microbiology and Infection
17
發行號3
DOIs
出版狀態已發佈 - 三月 2011

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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