Clathrin-mediated endocytosis is required for ANE 30-100K-induced autophagy

Mei Huei Lin, Young Chau Liu, Shyun Yeu Liu, Fang Chi Chen, Pei Jung Yang, Guan Hua Li, Pang Yen Liu, Ching Yu Yen

研究成果: 雜誌貢獻文章

4 引文 斯高帕斯(Scopus)

摘要

Background: We identified an autophagy-inducing areca nut (AN) ingredient (AIAI) in the 30-100 kDa fraction of AN extract (ANE 30-100K). This study was to analyze the role of endocytosis in ANE 30-100K-induced autophagy. Methods: We used benzyl alcohol, dynasore, and shRNA of clathrin and dynamin to assess whether ANE 30-100K-induced cytotoxicity and accumulation of microtubule-associated protein 1 light chain 3 (LC3)-II were affected in oral (OECM-1) and esophageal (CE81T/VGH) carcinoma cells. Results: Both benzyl alcohol and dynasore effectively reduced ANE 30-100K-induced cytotoxicity and LC3-II accumulation in OECM-1 and CE81T/VGH cells. Downregulated protein expression of both clathrin and dynamin by their shRNA also significantly attenuated ANE 30-100K-induced elevation of LC3-II levels in CE81T/VGH cells. Conclusions: These results indicate that AIAI may be engulfed by cells through clathrin-mediated endocytosis, which promotes the execution of the following autophagy program.
原文英語
頁(從 - 到)25-31
期刊Journal of Oral Pathology and Medicine
47
發行號1
DOIs
出版狀態已發佈 - 一月 2018

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Oral Surgery
  • Otorhinolaryngology
  • Cancer Research
  • Periodontics

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    Lin, M. H., Liu, Y. C., Liu, S. Y., Chen, F. C., Yang, P. J., Li, G. H., Liu, P. Y., & Yen, C. Y. (2018). Clathrin-mediated endocytosis is required for ANE 30-100K-induced autophagy. Journal of Oral Pathology and Medicine, 47(1), 25-31. https://doi.org/10.1111/jop.12593