Background: We identified an autophagy-inducing areca nut (AN) ingredient (AIAI) in the 30-100 kDa fraction of AN extract (ANE 30-100K). This study was to analyze the role of endocytosis in ANE 30-100K-induced autophagy. Methods: We used benzyl alcohol, dynasore, and shRNA of clathrin and dynamin to assess whether ANE 30-100K-induced cytotoxicity and accumulation of microtubule-associated protein 1 light chain 3 (LC3)-II were affected in oral (OECM-1) and esophageal (CE81T/VGH) carcinoma cells. Results: Both benzyl alcohol and dynasore effectively reduced ANE 30-100K-induced cytotoxicity and LC3-II accumulation in OECM-1 and CE81T/VGH cells. Downregulated protein expression of both clathrin and dynamin by their shRNA also significantly attenuated ANE 30-100K-induced elevation of LC3-II levels in CE81T/VGH cells. Conclusions: These results indicate that AIAI may be engulfed by cells through clathrin-mediated endocytosis, which promotes the execution of the following autophagy program.
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Oral Surgery
- Cancer Research
Lin, M. H., Liu, Y. C., Liu, S. Y., Chen, F. C., Yang, P. J., Li, G. H., Liu, P. Y., & Yen, C. Y. (2018). Clathrin-mediated endocytosis is required for ANE 30-100K-induced autophagy. Journal of Oral Pathology and Medicine, 47(1), 25-31. https://doi.org/10.1111/jop.12593