Cinnamomum osmophloeum extracts inhibit growth of Helicobacter pylori and postinfectious interleukin-8 expression in human gastric epithelial cells

Shiuh Bin Fang, Hsin Yu Ko, Sheng Tung Huang, Chih Hung Huang, Li Ting Li, Chia Chun Chen, Ke Chuan Wang, Cheng Pin Pai, Hung Chang Lee, Hsu Wei Fang

研究成果: 雜誌貢獻文章

2 引文 (Scopus)

摘要

Essential oils from leaves of Taiwan's indigenous cinnamon, Cinnamomum osmophloeum extracts (CEs), contain similar constituents to those of commercial cinnamons from dried barks of other Cinnamomum species. However, cinnamon bark extracts showed controversial in vitro and in vivo results in their antibacterial activities against H. pylori. Whether CEs inhibit H. pylori growth and inflammation of H. pylori-infected gastric epithelium is unknown. In this study, we used 4 h water distillation to obtain CEs, which comprised >95% trans-cinnamaldehyde and other 4 minor compounds that were confirmed by GC-MS and contained 1305.5 μg ml-1 of trans-cinnamaldehyde that was quantified by HPLC analysis. The minimal inhibitory concentration of trans-cinnamaldehyde in CEs against H. pylori was 40.8 μg ml-1 in 48 h co-cultures. We subsequently performed 2 cinnamon extract assays: 3 h treatment of CEs containing 6.3 μg ml-1 and 63 μg ml-1 of trans-cinnamaldehyde significantly inhibited IL-8 mRNA and protein expression in the 1 hour H. pylori-infected cells, whereas 6 h treatment of CEs containing 21 μg ml-1 of trans-cinnamaldehyde, but not CEs containing 10.5 μg ml-1 of trans-cinnamaldehyde, substantially downregulated IL-8 mRNA expression and these two CEs strongly suppressed IL-8 protein secretion in the 2 h H. pylori-infected cells. In the 2 h IL-1β-treated cells, both CEs and t-SSs containing 21 μg ml-1 and 10.5 μg ml-1 of trans-cinnamaldehyde for 6 h markedly downregulated IL-8 mRNA expression; CEs inhibited IL-8 protein production more potently than t-SSs when both contained the same concentration of trans-cinnamaldehyde at 21 μg ml-1. In conclusion, CEs concentration-dependently inhibit H. pylori growth and postinfectiously inhibit IL-8 mRNA and protein expression in H. pylori- and IL-1β-pretreated AGS cells. Moreover, CEs exhibited a stronger anti-inflammatory effect than trans-cinnamaldehyde, indicating that CEs can potentially be used to treat H. pylori infection.
原文英語
頁(從 - 到)22097-22105
頁數9
期刊RSC Advances
5
發行號28
DOIs
出版狀態已發佈 - 2015

指紋

Interleukin-8
Proteins
Essential oils
Messenger RNA
Distillation
Interleukin-1
Assays
Cells
Epithelial Cells
cinnamic aldehyde
Volatile Oils
Water
Anti-Inflammatory Agents

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Chemistry(all)

引用此文

Cinnamomum osmophloeum extracts inhibit growth of Helicobacter pylori and postinfectious interleukin-8 expression in human gastric epithelial cells. / Fang, Shiuh Bin; Ko, Hsin Yu; Huang, Sheng Tung; Huang, Chih Hung; Li, Li Ting; Chen, Chia Chun; Wang, Ke Chuan; Pai, Cheng Pin; Lee, Hung Chang; Fang, Hsu Wei.

於: RSC Advances, 卷 5, 編號 28, 2015, p. 22097-22105.

研究成果: 雜誌貢獻文章

Fang, Shiuh Bin ; Ko, Hsin Yu ; Huang, Sheng Tung ; Huang, Chih Hung ; Li, Li Ting ; Chen, Chia Chun ; Wang, Ke Chuan ; Pai, Cheng Pin ; Lee, Hung Chang ; Fang, Hsu Wei. / Cinnamomum osmophloeum extracts inhibit growth of Helicobacter pylori and postinfectious interleukin-8 expression in human gastric epithelial cells. 於: RSC Advances. 2015 ; 卷 5, 編號 28. 頁 22097-22105.
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title = "Cinnamomum osmophloeum extracts inhibit growth of Helicobacter pylori and postinfectious interleukin-8 expression in human gastric epithelial cells",
abstract = "Essential oils from leaves of Taiwan's indigenous cinnamon, Cinnamomum osmophloeum extracts (CEs), contain similar constituents to those of commercial cinnamons from dried barks of other Cinnamomum species. However, cinnamon bark extracts showed controversial in vitro and in vivo results in their antibacterial activities against H. pylori. Whether CEs inhibit H. pylori growth and inflammation of H. pylori-infected gastric epithelium is unknown. In this study, we used 4 h water distillation to obtain CEs, which comprised >95{\%} trans-cinnamaldehyde and other 4 minor compounds that were confirmed by GC-MS and contained 1305.5 μg ml-1 of trans-cinnamaldehyde that was quantified by HPLC analysis. The minimal inhibitory concentration of trans-cinnamaldehyde in CEs against H. pylori was 40.8 μg ml-1 in 48 h co-cultures. We subsequently performed 2 cinnamon extract assays: 3 h treatment of CEs containing 6.3 μg ml-1 and 63 μg ml-1 of trans-cinnamaldehyde significantly inhibited IL-8 mRNA and protein expression in the 1 hour H. pylori-infected cells, whereas 6 h treatment of CEs containing 21 μg ml-1 of trans-cinnamaldehyde, but not CEs containing 10.5 μg ml-1 of trans-cinnamaldehyde, substantially downregulated IL-8 mRNA expression and these two CEs strongly suppressed IL-8 protein secretion in the 2 h H. pylori-infected cells. In the 2 h IL-1β-treated cells, both CEs and t-SSs containing 21 μg ml-1 and 10.5 μg ml-1 of trans-cinnamaldehyde for 6 h markedly downregulated IL-8 mRNA expression; CEs inhibited IL-8 protein production more potently than t-SSs when both contained the same concentration of trans-cinnamaldehyde at 21 μg ml-1. In conclusion, CEs concentration-dependently inhibit H. pylori growth and postinfectiously inhibit IL-8 mRNA and protein expression in H. pylori- and IL-1β-pretreated AGS cells. Moreover, CEs exhibited a stronger anti-inflammatory effect than trans-cinnamaldehyde, indicating that CEs can potentially be used to treat H. pylori infection.",
author = "Fang, {Shiuh Bin} and Ko, {Hsin Yu} and Huang, {Sheng Tung} and Huang, {Chih Hung} and Li, {Li Ting} and Chen, {Chia Chun} and Wang, {Ke Chuan} and Pai, {Cheng Pin} and Lee, {Hung Chang} and Fang, {Hsu Wei}",
year = "2015",
doi = "10.1039/c4ra11026a",
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T1 - Cinnamomum osmophloeum extracts inhibit growth of Helicobacter pylori and postinfectious interleukin-8 expression in human gastric epithelial cells

AU - Fang, Shiuh Bin

AU - Ko, Hsin Yu

AU - Huang, Sheng Tung

AU - Huang, Chih Hung

AU - Li, Li Ting

AU - Chen, Chia Chun

AU - Wang, Ke Chuan

AU - Pai, Cheng Pin

AU - Lee, Hung Chang

AU - Fang, Hsu Wei

PY - 2015

Y1 - 2015

N2 - Essential oils from leaves of Taiwan's indigenous cinnamon, Cinnamomum osmophloeum extracts (CEs), contain similar constituents to those of commercial cinnamons from dried barks of other Cinnamomum species. However, cinnamon bark extracts showed controversial in vitro and in vivo results in their antibacterial activities against H. pylori. Whether CEs inhibit H. pylori growth and inflammation of H. pylori-infected gastric epithelium is unknown. In this study, we used 4 h water distillation to obtain CEs, which comprised >95% trans-cinnamaldehyde and other 4 minor compounds that were confirmed by GC-MS and contained 1305.5 μg ml-1 of trans-cinnamaldehyde that was quantified by HPLC analysis. The minimal inhibitory concentration of trans-cinnamaldehyde in CEs against H. pylori was 40.8 μg ml-1 in 48 h co-cultures. We subsequently performed 2 cinnamon extract assays: 3 h treatment of CEs containing 6.3 μg ml-1 and 63 μg ml-1 of trans-cinnamaldehyde significantly inhibited IL-8 mRNA and protein expression in the 1 hour H. pylori-infected cells, whereas 6 h treatment of CEs containing 21 μg ml-1 of trans-cinnamaldehyde, but not CEs containing 10.5 μg ml-1 of trans-cinnamaldehyde, substantially downregulated IL-8 mRNA expression and these two CEs strongly suppressed IL-8 protein secretion in the 2 h H. pylori-infected cells. In the 2 h IL-1β-treated cells, both CEs and t-SSs containing 21 μg ml-1 and 10.5 μg ml-1 of trans-cinnamaldehyde for 6 h markedly downregulated IL-8 mRNA expression; CEs inhibited IL-8 protein production more potently than t-SSs when both contained the same concentration of trans-cinnamaldehyde at 21 μg ml-1. In conclusion, CEs concentration-dependently inhibit H. pylori growth and postinfectiously inhibit IL-8 mRNA and protein expression in H. pylori- and IL-1β-pretreated AGS cells. Moreover, CEs exhibited a stronger anti-inflammatory effect than trans-cinnamaldehyde, indicating that CEs can potentially be used to treat H. pylori infection.

AB - Essential oils from leaves of Taiwan's indigenous cinnamon, Cinnamomum osmophloeum extracts (CEs), contain similar constituents to those of commercial cinnamons from dried barks of other Cinnamomum species. However, cinnamon bark extracts showed controversial in vitro and in vivo results in their antibacterial activities against H. pylori. Whether CEs inhibit H. pylori growth and inflammation of H. pylori-infected gastric epithelium is unknown. In this study, we used 4 h water distillation to obtain CEs, which comprised >95% trans-cinnamaldehyde and other 4 minor compounds that were confirmed by GC-MS and contained 1305.5 μg ml-1 of trans-cinnamaldehyde that was quantified by HPLC analysis. The minimal inhibitory concentration of trans-cinnamaldehyde in CEs against H. pylori was 40.8 μg ml-1 in 48 h co-cultures. We subsequently performed 2 cinnamon extract assays: 3 h treatment of CEs containing 6.3 μg ml-1 and 63 μg ml-1 of trans-cinnamaldehyde significantly inhibited IL-8 mRNA and protein expression in the 1 hour H. pylori-infected cells, whereas 6 h treatment of CEs containing 21 μg ml-1 of trans-cinnamaldehyde, but not CEs containing 10.5 μg ml-1 of trans-cinnamaldehyde, substantially downregulated IL-8 mRNA expression and these two CEs strongly suppressed IL-8 protein secretion in the 2 h H. pylori-infected cells. In the 2 h IL-1β-treated cells, both CEs and t-SSs containing 21 μg ml-1 and 10.5 μg ml-1 of trans-cinnamaldehyde for 6 h markedly downregulated IL-8 mRNA expression; CEs inhibited IL-8 protein production more potently than t-SSs when both contained the same concentration of trans-cinnamaldehyde at 21 μg ml-1. In conclusion, CEs concentration-dependently inhibit H. pylori growth and postinfectiously inhibit IL-8 mRNA and protein expression in H. pylori- and IL-1β-pretreated AGS cells. Moreover, CEs exhibited a stronger anti-inflammatory effect than trans-cinnamaldehyde, indicating that CEs can potentially be used to treat H. pylori infection.

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