Chrysin suppresses IL-6-induced angiogenesis via down-regulation of JAK1/STAT3 and VEGF: An in vitro and inzovo approach

Chiu Mei Lin, Kou-Gi Shyu, Bao Wei Wang, Hang Chang, Yen Hsu Chen, Jen Hwey Chiu

研究成果: 雜誌貢獻文章

38 引文 (Scopus)

摘要

Chrysin, 5,7-dihydroxyflavone, possesses many biologic properties. This study aimed to investigate the effects and molecular mechanisms of chrysin on IL-6-induced angiogenesis in vitro and in ovo. Chicken chorioallantoic membrane assay, an in ovo angiogenesis assay, showed chrysin significantly suppressed IL-6-induced neovascularization. Furthermore, chrysin significantly suppressed human umbilical vein endothelial cell (HUVECs) migration and tube formation. The signaling pathway involved in chrysin-related antiangiogenesis was also investigated. The data indicated that chrysin is able to down-regulate the expression of glycoprotein 130 (gp130), soluble IL-6 receptor (IL-6R), phosphorylated JAK1 and STAT3, and VEGF in HUVECs. The IL-6-induced binding of STAT3 was significantly suppressed by chrysin. Moreover, chrysin did not further suppress VEGF expression with STAT3 knocked down. Taken together, the results show that chrysin suppresses IL-6-induced angiogenesis through modulation of the sIL-6R/gp130/JAK1/STAT3/VEGF signaling pathway. Chrysin may provide new therapeutic potential for IL-6-induced pathological angiogenesis.
原文英語
頁(從 - 到)7082-7087
頁數6
期刊Journal of Agricultural and Food Chemistry
58
發行號11
DOIs
出版狀態已發佈 - 六月 9 2010

指紋

angiogenesis
interleukin-6
Vascular Endothelial Growth Factor A
Interleukin-6
Down-Regulation
glycoproteins
chorioallantoic membrane
Endothelial cells
Human Umbilical Vein Endothelial Cells
assays
cell movement
Assays
chrysin
In Vitro Techniques
Cytokine Receptor gp130
Pathologic Neovascularization
Interleukin-6 Receptors
Chorioallantoic Membrane
chickens
therapeutics

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

引用此文

Chrysin suppresses IL-6-induced angiogenesis via down-regulation of JAK1/STAT3 and VEGF : An in vitro and inzovo approach. / Lin, Chiu Mei; Shyu, Kou-Gi; Wang, Bao Wei; Chang, Hang; Chen, Yen Hsu; Chiu, Jen Hwey.

於: Journal of Agricultural and Food Chemistry, 卷 58, 編號 11, 09.06.2010, p. 7082-7087.

研究成果: 雜誌貢獻文章

Lin, Chiu Mei ; Shyu, Kou-Gi ; Wang, Bao Wei ; Chang, Hang ; Chen, Yen Hsu ; Chiu, Jen Hwey. / Chrysin suppresses IL-6-induced angiogenesis via down-regulation of JAK1/STAT3 and VEGF : An in vitro and inzovo approach. 於: Journal of Agricultural and Food Chemistry. 2010 ; 卷 58, 編號 11. 頁 7082-7087.
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abstract = "Chrysin, 5,7-dihydroxyflavone, possesses many biologic properties. This study aimed to investigate the effects and molecular mechanisms of chrysin on IL-6-induced angiogenesis in vitro and in ovo. Chicken chorioallantoic membrane assay, an in ovo angiogenesis assay, showed chrysin significantly suppressed IL-6-induced neovascularization. Furthermore, chrysin significantly suppressed human umbilical vein endothelial cell (HUVECs) migration and tube formation. The signaling pathway involved in chrysin-related antiangiogenesis was also investigated. The data indicated that chrysin is able to down-regulate the expression of glycoprotein 130 (gp130), soluble IL-6 receptor (IL-6R), phosphorylated JAK1 and STAT3, and VEGF in HUVECs. The IL-6-induced binding of STAT3 was significantly suppressed by chrysin. Moreover, chrysin did not further suppress VEGF expression with STAT3 knocked down. Taken together, the results show that chrysin suppresses IL-6-induced angiogenesis through modulation of the sIL-6R/gp130/JAK1/STAT3/VEGF signaling pathway. Chrysin may provide new therapeutic potential for IL-6-induced pathological angiogenesis.",
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AB - Chrysin, 5,7-dihydroxyflavone, possesses many biologic properties. This study aimed to investigate the effects and molecular mechanisms of chrysin on IL-6-induced angiogenesis in vitro and in ovo. Chicken chorioallantoic membrane assay, an in ovo angiogenesis assay, showed chrysin significantly suppressed IL-6-induced neovascularization. Furthermore, chrysin significantly suppressed human umbilical vein endothelial cell (HUVECs) migration and tube formation. The signaling pathway involved in chrysin-related antiangiogenesis was also investigated. The data indicated that chrysin is able to down-regulate the expression of glycoprotein 130 (gp130), soluble IL-6 receptor (IL-6R), phosphorylated JAK1 and STAT3, and VEGF in HUVECs. The IL-6-induced binding of STAT3 was significantly suppressed by chrysin. Moreover, chrysin did not further suppress VEGF expression with STAT3 knocked down. Taken together, the results show that chrysin suppresses IL-6-induced angiogenesis through modulation of the sIL-6R/gp130/JAK1/STAT3/VEGF signaling pathway. Chrysin may provide new therapeutic potential for IL-6-induced pathological angiogenesis.

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