Chrysin inhibits lipopolysaccharide-induced angiogenesis via down-regulation of VEGF/VEGFR-2(KDR) and IL-6/IL-6R pathways

Chiu Mei Lin, Hang Chang, Shih Yun Li, I. Hsing Wu, Jen Hwey Chiu

研究成果: 雜誌貢獻文章

24 引文 (Scopus)

摘要

The relationship between chrysin and inflammation-induced angiogenesis remains unclear. The aim of this study was to evaluate the suppressive effects of chrysin on lipopolysaccharide (LPS)-induced angiogenesis in chicken chorioallantoic membrane (CAM) as well as in human umbilical endothelial cells (HUVEC). The in vivo CAM model was applied to evaluate the percentage of new vessels formation, followed by measuring endothelial migration and tube formation in HUVEC cultures. The mechanisms of the suppressive effect of chrysin on LPS-induced angiogenesis, in terms of VEGF, VEGF receptors (VEGFR), interleukin 6 (IL-6) and IL-6 receptor gene expressions, were analyzed by Western blot, ELISA cytokine assay, and quantitative real time PCR. The results showed that chrysin (10-8-10-5M) inhibited LPS-induced CAM neovascular density. There was a significant down-regulation of VEGF and VEGFR-2 (KDR) but not VEGFR-1 (Flt-1) gene expression by chrysin in LPS-treated HUVEC cultures. Besides, chrysin concentration-dependently inhibited the auto-regulation loop of IL-6/IL-6R in LPS-treated HUVEC cells. We conclude that chrysin suppresses both in vitro and in vivo LPS-induced angiogenesis.

原文英語
頁(從 - 到)708-714
頁數7
期刊Planta Medica
72
發行號8
DOIs
出版狀態已發佈 - 六月 2006

指紋

vascular endothelial growth factor receptors
Vascular Endothelial Growth Factor Receptor-2
Vascular Endothelial Growth Factor Receptor
angiogenesis
interleukin-6
lipopolysaccharides
Vascular Endothelial Growth Factor A
Lipopolysaccharides
Interleukin-6
Down-Regulation
chorioallantoic membrane
Chorioallantoic Membrane
Membranes
Gene expression
umbilicus
autoregulation
gene expression
Interleukin-6 Receptors
Umbilicus
Gene Expression

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

引用此文

Chrysin inhibits lipopolysaccharide-induced angiogenesis via down-regulation of VEGF/VEGFR-2(KDR) and IL-6/IL-6R pathways. / Lin, Chiu Mei; Chang, Hang; Li, Shih Yun; Wu, I. Hsing; Chiu, Jen Hwey.

於: Planta Medica, 卷 72, 編號 8, 06.2006, p. 708-714.

研究成果: 雜誌貢獻文章

Lin, Chiu Mei ; Chang, Hang ; Li, Shih Yun ; Wu, I. Hsing ; Chiu, Jen Hwey. / Chrysin inhibits lipopolysaccharide-induced angiogenesis via down-regulation of VEGF/VEGFR-2(KDR) and IL-6/IL-6R pathways. 於: Planta Medica. 2006 ; 卷 72, 編號 8. 頁 708-714.
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abstract = "The relationship between chrysin and inflammation-induced angiogenesis remains unclear. The aim of this study was to evaluate the suppressive effects of chrysin on lipopolysaccharide (LPS)-induced angiogenesis in chicken chorioallantoic membrane (CAM) as well as in human umbilical endothelial cells (HUVEC). The in vivo CAM model was applied to evaluate the percentage of new vessels formation, followed by measuring endothelial migration and tube formation in HUVEC cultures. The mechanisms of the suppressive effect of chrysin on LPS-induced angiogenesis, in terms of VEGF, VEGF receptors (VEGFR), interleukin 6 (IL-6) and IL-6 receptor gene expressions, were analyzed by Western blot, ELISA cytokine assay, and quantitative real time PCR. The results showed that chrysin (10-8-10-5M) inhibited LPS-induced CAM neovascular density. There was a significant down-regulation of VEGF and VEGFR-2 (KDR) but not VEGFR-1 (Flt-1) gene expression by chrysin in LPS-treated HUVEC cultures. Besides, chrysin concentration-dependently inhibited the auto-regulation loop of IL-6/IL-6R in LPS-treated HUVEC cells. We conclude that chrysin suppresses both in vitro and in vivo LPS-induced angiogenesis.",
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AU - Wu, I. Hsing

AU - Chiu, Jen Hwey

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AB - The relationship between chrysin and inflammation-induced angiogenesis remains unclear. The aim of this study was to evaluate the suppressive effects of chrysin on lipopolysaccharide (LPS)-induced angiogenesis in chicken chorioallantoic membrane (CAM) as well as in human umbilical endothelial cells (HUVEC). The in vivo CAM model was applied to evaluate the percentage of new vessels formation, followed by measuring endothelial migration and tube formation in HUVEC cultures. The mechanisms of the suppressive effect of chrysin on LPS-induced angiogenesis, in terms of VEGF, VEGF receptors (VEGFR), interleukin 6 (IL-6) and IL-6 receptor gene expressions, were analyzed by Western blot, ELISA cytokine assay, and quantitative real time PCR. The results showed that chrysin (10-8-10-5M) inhibited LPS-induced CAM neovascular density. There was a significant down-regulation of VEGF and VEGFR-2 (KDR) but not VEGFR-1 (Flt-1) gene expression by chrysin in LPS-treated HUVEC cultures. Besides, chrysin concentration-dependently inhibited the auto-regulation loop of IL-6/IL-6R in LPS-treated HUVEC cells. We conclude that chrysin suppresses both in vitro and in vivo LPS-induced angiogenesis.

KW - Angiogenesis

KW - Chrysin

KW - KDR

KW - Lipopolysaccharide

KW - VEGF

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