摘要

Objective. Few studies have investigated the feasibility of using chronic pudendal neuromodulation for improving voiding function in patients with diabetes who are also experiencing urinary retention. The present study investigated the effects of chronic electrical stimulation (ES) of the sensory branch of the pudendal nerve on voiding function in diabetic rats. Approach. A custom-made implantable microstimulation system was designed and manufactured for chronic implantation in normal control (NC) and diabetic rats. After three or six weeks of pudendal neuromodulation, the intravesical pressure, external urethral sphincter electromyograms (EUS-EMGs), and urine flow rate (UFR) of all rats were simultaneously recorded to assess the effects of chronic pudendal ES on voiding function. Morphological changes in pudendal axons were assessed through hematoxylin and eosin (H&E) staining. Results. Abnormal cystometric measurements, including an increased volume threshold, contraction amplitude, and residual volume (RV) as well as a decreased burst period (BP) indicated voiding dysfunction in the diabetic rats with sham ES for three or six weeks. However, real ES for three or six weeks reversed these abnormal results; these parameters became similar to those of the NC rats and, consequently, voiding efficiency (VE) increased significantly from 17%-45% to 51%-57%. The cross-sectional area and axonal density of the pudendal nerve decreased in all the diabetic rats with sham ES for six weeks; however, these values increased significantly after real ES for six weeks. Significance. This study demonstrated the feasibility of using chronic pudendal neuromodulation for improving voiding function in diabetic rats. These results may facilitate the development of an advanced neural prosthesis for restoring bladder function in clinical settings.

原文英語
文章編號046001
期刊Journal of Neural Engineering
13
發行號4
DOIs
出版狀態已發佈 - 五月 17 2016

指紋

Rats
Electric Stimulation
Pudendal Nerve
Neural prostheses
Rat control
Neural Prostheses
Medical problems
Residual Volume
Urinary Retention
Feasibility Studies
Electromyography
Urethra
Hematoxylin
Eosine Yellowish-(YS)
Axons
Flow rate
Urinary Bladder
Urine
Staining and Labeling
Pressure

ASJC Scopus subject areas

  • Biomedical Engineering
  • Cellular and Molecular Neuroscience

引用此文

Chronic pudendal neuromodulation using an implantable microstimulator improves voiding function in diabetic rats. / Hsieh, Tsung Hsun; Lin, Yin Tsong; Chen, Shih Ching; Peng, Chih Wei.

於: Journal of Neural Engineering, 卷 13, 編號 4, 046001, 17.05.2016.

研究成果: 雜誌貢獻文章

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abstract = "Objective. Few studies have investigated the feasibility of using chronic pudendal neuromodulation for improving voiding function in patients with diabetes who are also experiencing urinary retention. The present study investigated the effects of chronic electrical stimulation (ES) of the sensory branch of the pudendal nerve on voiding function in diabetic rats. Approach. A custom-made implantable microstimulation system was designed and manufactured for chronic implantation in normal control (NC) and diabetic rats. After three or six weeks of pudendal neuromodulation, the intravesical pressure, external urethral sphincter electromyograms (EUS-EMGs), and urine flow rate (UFR) of all rats were simultaneously recorded to assess the effects of chronic pudendal ES on voiding function. Morphological changes in pudendal axons were assessed through hematoxylin and eosin (H&E) staining. Results. Abnormal cystometric measurements, including an increased volume threshold, contraction amplitude, and residual volume (RV) as well as a decreased burst period (BP) indicated voiding dysfunction in the diabetic rats with sham ES for three or six weeks. However, real ES for three or six weeks reversed these abnormal results; these parameters became similar to those of the NC rats and, consequently, voiding efficiency (VE) increased significantly from 17{\%}-45{\%} to 51{\%}-57{\%}. The cross-sectional area and axonal density of the pudendal nerve decreased in all the diabetic rats with sham ES for six weeks; however, these values increased significantly after real ES for six weeks. Significance. This study demonstrated the feasibility of using chronic pudendal neuromodulation for improving voiding function in diabetic rats. These results may facilitate the development of an advanced neural prosthesis for restoring bladder function in clinical settings.",
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