Chorioallantoic membrane assays have been based on diffusion control-Problems arising with a diversity of mass transfers in egg white

Chiu Lan Hsieh, Chiung Chi Peng, Li Yun Lin, Yu Ting Cheng, Kuan Chou Chen, Robert Y. Peng

研究成果: 雜誌貢獻文章同行評審

摘要

The chorioallantoic membrane (CAM) assays have been intensively used to determine angiogenesis and anti-angiogenesis of medicines. In view of bioactivity, this technique should be performed with kinetic control regime in chicken embryos. Whether the dosages ever used had satisfied this requirement, we explored by mathematical analysis. A diffusion-in-egg model was established to describe several medicinal diffusions in egg white that involved the instantaneous transient kinetic behavior, the diffusion of medicines in capping volume (the volume from the air sac to the interface of egg yolk). By reviewing the diffusion of various compounds including the cited and the experimentals in this work, we conclude that all the CAM assays ever cited were performed under diffusion control regime rather than kinetic control, which may bring forth deviations caused by a diversity of constitutes in egg white through various medicine-protein interactions.
原文英語
頁(從 - 到)74-78
頁數5
期刊Colloids and Surfaces B: Biointerfaces
68
發行號1
DOIs
出版狀態已發佈 - 一月 1 2009

ASJC Scopus subject areas

  • 生物技術
  • 膠體和表面化學
  • 物理與理論化學
  • 表面和介面

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